Unilever Patent Holdings B.v.

Hein Stam

Wouter Musters

Johannes M Verbakel

Jacob B Visser

Maria E Suykerbuyk

Jan Maat

Robertus Johannes Gouka

Peter J Schaap

Cornelis Antonius Van Den Hondel

Peter J Van De Vonderwoort

Process for producing/secreting a protein by a transformed mould using

The invention relates to isolation of an Aspergillus gene encoding rhamnogalacturonase (RG-ase) and the construction of recombinant Aspergillus strains with overexpression of RG-ase. These strains can be used for the commercial production of RG-ase. RG-ase is an important enzyme in processes requiring the degradation and/or modification of pectin or modification of pectin-containing vegetable or plant cell wall material. RG-ase may be used in various applications, including the processing of fruits and vegetables, in the extraction of components from vegetable material or for improving the functionality of pectin or pectin-containing vegetable material, food material or plant cell wall material.

Methods of detecting and isolating a ripening form of a polypeptide

Cloning and expression of DNA encoding a ripening form of a polypeptide

The invention relates to recombinant DNA technology for the production of an enzyme having sulfhydryl oxidase ('SOX') activity. This SOX-enzyme can be used where the oxidation of free sulfhydryl groups (thio compounds) to the corresponding disulfides is desirable. SOX enzyme may be used for treatment of bakery products or for removal of off-flavour from milk or beer.

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Diemen, Netherlands

Hein Stam