Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V.

Universitaet Des Saarlandes

Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Universität Des Saarlandes

Institut Fur Bioprozess-und Analysenmesstechnik Ev

Helmholtz Zentrum Munchen Deutsches Forschungszentrum Fuer Gesundheit Und Umwelt

Heiko Zimmermann

Guenter R Fuhr

Günter R Fuhr

Julia Neubauer

Rolf Hagedorn

Frank Stracke

Gunter Fuhr

Ulrich Zimmermann

Uwe Schön

Charli Kruse

Jörg-Uwe Meyer

Ronan Le Harzic

Young-Joo Oh

Hagen Thielecke

Christian Degel

Hagen Von Briesen

Robert Lemor

Michael Gepp

Benjamin Fischer

Thomas Fixemer

Thomas Trautmann

Philippe Bastiaens

Marianne Belke

Dirk Leuthold

Uwe Schoen

Frank Ihmig

Stephen Shirley

Klaus-Peter Hoffmann

Anja Germann

Axel Beier

Daniel Doerr

Till Brombach

Günther R Fuhr

Daniel DÖRR

Karim Hichri

Friederike Ehrhart

Michael Möller

Günther Fuhr

Josef Metze

Tomm Schmidt

Bernd Kranz

Gunter R Fuhr

Haiko Wick

Markus Grabenbauer

Frank Wehner

Gunter Gastrock

Antonie Fuhr

Günter Fuhr

The invention relates to a low temperature indicator mixture for indicating/monitoring a temperature transition within a temperature range of between −20° C. and −160° C., which comprises at least one dye, e.g. oil red, methyl rot, brilliant green, rhodamine B, and at least one alcohol selected from the group that comprises octane-1-ol, nonan-1-ol, propane-1,2-diol, propane-1,3-diol, butane-1,2-diol, butane-1,3-diol, butane-2-ol, pentane-1,5-diol, pentane-1-ol, cyclopentanol, benzyl alcohol, wherein the melt temperature of the mixture is within the aforementioned temperature range. Further aspects of the invention relate to devices and methods for indicating/monitoring a temperature transition within the aforementioned temperature range, in which devices and methods the aforementioned low temperature indicator mixture is used. In a specific embodiment, a device of this kind comprises at least the following components in at least one closed space: a) a partial region, which is at least partially filled with an indicator mixture as defined above; b) a second partial region, which is at least partially filled with an absorbent material, said material being optionally separated from the indicator mixture by a separating element; and c) optionally means to penetrate the separating element to bring the indicator mixture in a liquid state into contact with the absorbent material. In a method according to the invention for temperature monitoring using a device of this kind, a check is carried out to determine whether at least part of the indicator mixture was absorbed by the absorbent material, which indicates an at least temporary increase in the temperature of the device to at least the melt temperature of the indicator mixture.

Low temperature indicator mixture, device and method for monitoring a temperature transition at low temperatures

The invention relates to a method for producing three-dimensional, preferably porous, hydrogel structures by means of layer build-up technique, wherein the method comprises the following steps. Providing (S) of a liquid hydrogel solution, preferably a liquid alginate solution, and a, preferably transportable, sample carrier. Layerwise applying (S) the liquid hydrogel solution onto the sample carrier in a temperature environment, the temperature of which is below the freezing point of the hydrogel solution, to produce a frozen 3D layered hydrogel structure. In order to increase advantageously the porosity of the 3D layered hydrogel structure, i.e. the proportion of small voids, cavities and/or depressions in the 3D layered hydrogel structure, the method according to the invention further comprises the step of drying (S) of the frozen 3D layered hydrogel structure, e.g. by means of freeze-drying, to produce a porous 3D hydrogel structure. The invention relates further also to a device for the layerwise building-up of three-dimensional hydrogel structures.

Method for producing three-dimensional hydrogel structures and device for the layerwise building-up of such hydrogel structures

The invention relates to a device for the temperature monitoring of a cryopreserved biological sample, comprising a sample container (in particular a cryotube) having a holding space for holding a biological sample and comprising at least one chamber, the interior of which is not fluidically connected to the holding space and is filled only partially with an indicator substance, the melting temperature of which lies in a range of &#x2212;20&#xb0; C. to &#x2212;140&#xb0; C. In particular, the chamber can be formed by a container that is detachably or pivotably fastened to the sample container. Alternatively, the chamber is formed by a double-walled slide-on part or the holding space of the sample container is double-walled, wherein an intermediate space between the inner wall and the outer wall is partially filled with the indicator substance.

Method and device for the temperature monitoring of a cryopreserved biological sample

The invention relates to a method for monitoring the temperature of a cryopreserved biological sample. The invention also relates to a device for monitoring the temperature of a cryopreserved biological sample. The device () for monitoring the temperature of a cryopreserved biological sample comprises a sample container () having a receiving space () for receiving a biological sample (). The device also comprises at least one chamber () having an interior that is not fluidically connected to the receiving space () and is only partially filled with an indicator substance () with a melting temperature in a region of &#x2212;20&#xb0; C. to &#x2212;140&#xb0; C. The chamber () has a barrier () that causes the indicator substance () to move into a second sub-region () of the chamber () when the indicator substance () in a first sub-region () of the chamber is in the fluid aggregate state.

Device and method for monitoring the temperature of a cryopreserved biological sample

The invention relates to a device for the temperature monitoring of a cryopreserved biological sample, comprising a sample container having an accommodating space (2) for accommodating the sample and comprising an indicating apparatus, which can be arranged on the outside of the sample container, for monitoring at least one temperature limit value. The indicating apparatus has at least one cavity, which is only partially filled with an indicating substance, the melting temperature of which lies in a range from &#x2212;20 &#xb0; C. to &#x2212;140 &#xb0; C. The indicating apparatus can be designed, in particular, as a cylindrical body, which can be fastened to a cryotube as a bottom part, or alternatively as a double-walled hollow cylinder, which can be slid onto an outer lateral surface of the cryotube. The indicating apparatus can also be fastened to a lateral outer wall of the sample container, e.g. as a hollow body that can be inserted into a sleeve or insertion pocket.

The invention relates to a device for monitoring the temperature of a cryogenically preserved biological sample. The device () comprises a sample container () having a receptacle space () for accommodating a biological sample (). The device further comprises at least one chamber (), the interior () of which is not fluidically connected to the receptacle space, and which is at least partially filled with an indicator substance () having a boiling point or sublimation temperature in a range from &#x2212;10&#xb0; C. to &#x2212;140&#xb0; C. The chamber () further has at least one opening via which the indicator substance () can escape from the interior () of the chamber () upon exceeding its boiling point or sublimation point. The invention further relates to a method for monitoring the temperature of a cryogenically preserved biological sample.

Device and method for monitoring the temperature of a cryogenically preserved biological sample

The invention relates to a temperature-control element () for a multiwell plate (), which comprises a plurality of cavities () arranged in rows and columns for freezing and/or thawing biological samples. The temperature-control element () comprises a base body () which is made of a thermally conductive material and is flown through by a temperature-control fluid; and a plurality of protruding temperature-control fingers () arranged in rows and columns on an upper side of the base body (), which are connected in a thermally conductive manner to the base body (), wherein a grid spacing of the temperature control fingers () corresponds to a grid spacing of the cavities () of the multiwell plate (). The invention further relates to a device and method for freezing biological samples, in particular for cryopreservation, and/or thawing biological samples, in particular a cryopreserved sample.

Temperature-control element for a multiwell plate and method and device for freezing and/or thawing biological samples

The invention relates to a sample collection kit () for collecting plant samples, in particular algae samples for biomedical applications, which sample collection kit comprises at least one sample container for holding plant samples, a plurality of sensors, by means of which sample data can be captured, a data-recording apparatus for recording the sample data, an arrangement of tools, which has collection tools designed for harvesting the plant samples and processing tools designed for processing the plant samples, an arrangement of devices, which has auxiliary devices that can be used in the collection, transport, and processing of the plant samples, a container apparatus (), which is designed to hold the arrangement of tools and the arrangement of devices in isolation from the environment, and identification markings (), which contain identification data and are applied to the at least one sample container () to the collection tools, and to the processing tools. The invention further relates to a sample collection method.

Sample collection kit and method for collecting plant samples, in particular algae samples

The invention relates to a method and a device for optical in vitro detection of a movement in a biological sample with a spatial extent in the form of a three-dimensional cell and/or tissue culture or a cell cluster or a sample made of freely swimming microorganisms. The method comprises the following steps: (a) providing a receptacle for the sample (), a light beam source (), an optical unit () and a detector (), (a1) wherein the optical unit () is embodied to illuminate the whole sample () in the receptacle with radiation emanating from the light beam source and to guide at least some of the radiation () of the light beam source (), which is changed at any point within the sample () by an interaction with the sample () in terms of the beam direction thereof, the polarization state thereof and/or the diffraction pattern thereof, to a detection surface () of the detector (), and (a2) wherein the detector () is embodied to generate a measurement signal () in a manner dependent on the detected radiation, the time profile of which measurement signal specifies a time profile of an intensity of the detected radiation () and/or from which the time profile of the intensity of the detected radiation () is derivable; (b) illuminating the sample () with radiation from the light beam source; and (c) detecting a movement in the biological sample () in a manner dependent on a temporal change in the measurement signal ().

Method and device for optical detection of a movement in a biological sample with a spatial extent

The invention relates to a method and a device for the optical in vitro detection of a movement in a biological sample and/or for the optical in vitro detection of a movement of a component of the biological sample. The method has the following step: (a) providing an optical wide-field illumination device for illuminating the sample, said device being designed to illuminate the entire sample, and a detector () for detecting radiation () coming from the sample. The detector () has a detection surface () which is divided into multiple detection regions (). The detector is additionally designed to derive (S) detection signals () of individual detection regions () with respect to time, subsequently rectify (S) the signals, preferably by generating an absolute value or squaring, and summing or averaging (S) the derived and rectified detection signals of all of the detection regions and then providing same as an output signal (). The method further has the steps of illuminating the sample using the wide-field illumination device and detecting a movement in the biological sample on the basis of the output signal () of the detector ().

Method and device for optically detecting a movement in a biological sample

A culture vessel (), which is designed for culturing biological cells () in hanging drops (), comprises a vessel wall (), which has a cover portion () and a floor portion (), wherein the cover portion () is designed to provide a culturing surface (), and the floor portion () is designed to receive a liquid (), the vessel wall () encloses an interior () of the culture vessel on all sides, the culturing surface () has holding elements (), which are designed to position the drops (), wherein the culturing surface () can receive the drops () hanging freely in the interior (), and the vessel wall () is movable such that the holding elements () can be wetted with the liquid () from the floor portion (). A method for culturing biological cells () in hanging drops () in the culture vessel () is also described.

Culture vessel and method for culturing biological cells in hanging drops

The invention relates to an encapsulating device (), which is designed to encapsulate a sample () in a polymer capsule, comprising a drop generator (), which is designed to provide a drop () of a suspension, which drop contains the sample (), and a cross-linking device (), which is designed to polymerize the drop (), wherein the drop generator () has a retaining device (), which is designed to accommodate the drop () in a hanging state, and the cross-linking device () is designed to feed a polymerization substance to the hanging drop () on the retaining device () and to form the polymer capsule. The invention further relates to a method for encapsulating a sample () in a polymer capsule.

Encapsulating device and encapsulating method for encapsulating a sample in a polymer capsule

The invention relates to a method for monitoring a cryopreserved biological sample (), comprising the steps of providing the biological sample () in a cryopreserved state, measuring at least one Raman spectroscopic sample characteristic by means of a Raman spectroscopic measuring apparatus (), comparing the at least one sample characteristic with a reference characteristic by means of an evaluating apparatus (), and providing a state characteristic that depends on the result of the comparison and that is characteristic of a storage state of the biological sample (). The invention further relates to a monitoring device for cryopreserved samples, in particular for performing said method.

Method and device for monitoring a cryopreserved biological sample

A substrate unit for cryopreservation of a biological sample includes: a substrate platform having on a front side thereof a cultivation surface for receiving the biological sample; a first chamber including the cultivation surface of the substrate platform, wherein the first chamber is configured for receiving a cultivation liquid; a second chamber configured for receiving a temperature control medium; and a chamber bracket for receiving the substrate unit in a cryopreservation device in a pivotable manner, wherein the first and second chambers are connected to each other in an adjacent manner, and the substrate platform forms a separating wall between the first chamber and the second chamber, and wherein the back side of the substrate platform faces the second chamber. The invention further relates to a cryopreservation device, wherein the substrate unit is arranged in a rotatable manner, and a method for the cryopreservation of a biological sample.

Substrate unit, preservation device and method for the cryopreservation of a biological sample

The invention relates to a cooling apparatus, especially for cryogenically preserving biological samples, including a duct () for delivering a coolant () to a cooling chamber (), a heater () that has an adjustable first heating performance (P) for heating the coolant () delivered to the cooling chamber (), a first temperature sensor (-) for measuring the temperature (T-T) in the cooling chamber (), a second temperature sensor () for measuring the temperature (Ti) of the coolant () delivered to the cooling chamber (I), and a regulator () for regulating the temperature. The regulator () is embodied as a multiple regulator which detects several temperatures (T-T) as control variables and/or adjusts several heating performances (P, P) as manipulated variables. The invention further relates to a corresponding operating method.

Cooling apparatus used for cryonic preservation, and corresponding operating method

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St. Ingbert, Germany

Heiko Zimmermann