Algenol Biotech LLC

Algenol Biofuels, Inc.

Cyano Biotech Gmbh

Algenol Biofuels Switzerland Gmbh

Heike Enke

Ulf Dühring

Kerstin Baier

Dan Kramer

Alexandra Friedrich

Karl Ziegler

Frank Uliczka

Kui Wang

Irina Piven

Tuo Shi

Masami Inaba

Torsten Schwecke

Stefan Jahns

Wolfram Lorenzen

Timo Niedermeyer

Ulf Duhring

Dan Enke

Julia Moschny

Christine Oesterhelt

Benjamin A McCool

Paul Gordon Roessler

Ming-De Deng

Benjamin Moll

Karola Knuth

R Paul Woods

John Robert Coleman

Juliane Metzner

William Drake

Ryan Adams

William Porubsky

Ulf Duehring

Wolfgang Lockau

Marianne Grundel

Craig R Smith

Frank Jochem

Christian Weissert

Songhua Zhu

Charles Ryan Budinoff

Ulf DÜHRING

A compound includes cytotoxic agents such as microcystin and nodularin. More specifically, a modified microcystin and/or nodularin compound contains one or more modified substrates, wherein one or more modified substrates include an anchor group directly accessible or transformable for use in conjugation chemistry (incl. click chemistry), for the attachment of a targeting moiety. This compound is useful for cancer treatment.

Modified microcystins and nodularins

A method is used for producing a modified non-ribosomal peptide, e.g. a modified microcystin and/or modified nodularin (together CA), including the steps of: a) growing a modified non-ribosomal peptide producing cyanobacteria strain in a culture media, h) adding one or more modified substrates, preferably modified amino acids to said culture, and c) inoculating the non-ribosomal peptide, producing strain the presence of said modified substrates. The thus modified non-ribosomal peptide may be used for the therapy of various diseases.

Method for modifying microcystins and nodularins

The invention provides genetically modified cyanobacterial cells that are capable of utilizing phosphite as a primary phosphorus source, and can out-compete contaminant organisms for certain forms of phosphorus more effectively.

Cyanobacterial strains capable of utilizing phosphite

A genetically enhanced cyanobacterial host cell,sp. ABICyanoI, is disclosed. The enhancedsp. ABICyanoI produces a compound or compounds of interest.

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This invention provides a method for the prolonged production of ethanol using a metabolically enhanced cyanobacterium.

Metabolically enhanced cyanobacterium with sequentially inducible production genes for the production of a first chemical compound

The invention provides novel compositions of matter for the production of ethanol from carbon dioxide and water. Particularly, the invention provides photoautotrophic organisms having a first and second genetic modification, wherein the first genetic modification improves the ethanol production from organisms having the second genetic modification.

Genetically modified cyanobacteria for the production of ethanol

A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zndependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced, a method for producing ethanol with the metabolically enhanced, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.

Metabolically enhanced cyanobacterial cell for the production of ethanol

One embodiment of the invention provides a genetically enhancedsp. host cell comprising at least one first recombinant gene encoding a first protein for the production of ethanol under the transcriptional control of a first inducible promoter, having at least 85%, 90% or 95% sequence identity to an endogenous inducible promoter of thesp. host cell.

This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds. Further provisions of the present invention include, inter alia, a method for producing the metabolically enhanced cyanobacterium and a method for producing a first chemical compound by culturing the metabolically enhanced cyanobacterium.

A cyanobacterial host cell,sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.

A genetically enhanced cyanobacterial host cell,sp. ABICyano1, is disclosed. The enhancedsp. ABICyano1 produces a compound or compounds of interest.

A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zndependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.

Flat panel biofilm photobioreactor systems with a photosynthetic, autofermentative microorganism that forms a biofilm and methods for using the same to make metabolic intermediate compound(s) through photosynthesis and to convert metabolic intermediate compound(s) into chemical product(s) such as a biofuel or a feedstock through autofermentation.

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Berlin, Germany

Heike Enke