Siemens Aktiengesellschaft

Boehringer Ingelheim Vetmedica Gmbh

Heike Barlag

Walter Gumbrecht

Manfred Stanzel

Konrad Mund

Siegfried Birkle

Peter Paulicka

Jörg Heuer

Jochen Ostermaier

Markus Böhm

Daniela Kühn

A method is disclosed for charging at least one energy store of an electric vehicle via a charging point, using a first terminal having first connecting elements of a charging cable connected to the charging point. The first connecting elements are connected to corresponding second connecting elements of a second terminal on the vehicle, and the first and second connecting elements are designed to supply alternating current for charging the at least one energy store. Direct current from the charging point for charging the at least one energy store is supplied to the electric vehicle via at least two first connecting elements or at least two third connecting elements of the first terminal, wherein PLC communication between the electric vehicle and charging point in order to exchange information relating to the charging operation occurs via one or more first connecting elements on which no direct current for charging is carried.

Method for charging an electric vehicle by supplying DC over a cable configured for AC charging

A device is intended to electrochemically measure biochemical reactions and includes a base plate, a sensor array situated on the latter, a coating of the base plate, and a sealing film with at least one recess. The recess is mechanically connected to the base plate and/or to the coating of the base plate and forms a flow cell above the sensor array. An inlet and an outlet of the flow cell are in the form of continuous recesses in the base plate. The active surfaces of the sensors are free of the coating and regions of the base plate adjacent to the sensors are covered by the coating.

Arrangement and method for electrochemically measuring biochemical reactions and method for producing the arrangement

Electrochemical transducer arrays are already known from the prior art. According to the invention, the transducer array is provided with at least one flexible, planar metal substrate on which at least one flexible insulator having a firm connection between the metal surface and the insulator surface is disposed. The metal substrate and the insulator disposed thereon are structured in such a manner as to give metal areas which are electrically insulated the one from the other and which serve as sensor areas. The metal substrate used is self-contained so that the structured metal areas can be contacted from the lower side.

Electrochemical transducer array and use thereof

A method follow the change in concentration of a redox-active substance, whereby suitable potentials for a reduction process or an oxidation process are applied to the working electrode of a measuring device. The potential of the working electrode is pulsed and measuring phases and relaxation phases are alternately produced, the pulse lengths of measuring phase and relaxation phase being determined in a suitable manner. A rapid relaxation of the concentration gradient is electrochemically forced so that the measurement can be carried out on simple transducer arrays. A device for carrying out the method includes a transducer array in addition to a suitable potentiostat. The transducer array can be formed of a planar metal substrate on which at least one flexible insulator is disposed, the metal surface and the insulator surface being firmly linked. The transducer array can also be formed of silicone-based CMOS structures.

Method and system for determining the concentration of an analyte/enzyme complex or of an analyte/enzyme conjugate, especially for the electrochemical detection of the analyte, and corresponding measuring device

In order to follow the change in concentration of a redox-active substance, potential suitable for a reducing process or oxidation process are applied to the working electrode of a measuring device. The potential of the working electrode is pulsed and measuring phases and relaxation phases are alternately produced, the pulse lengths of the measuring phase and relaxation phase being predetermined in a suitable manner. In this manner, a rapid relaxation of the concentration gradient is forced electrochemically so that the measurement can be carried out on simple transducer arrays. The device includes a transducer array in addition to a suitable potentiostat. The transducer array may include a planar metal substrate on which at least one flexible insulator having a firm connection between the metal surface and the insulator surface is located. The array is generated by suitably structuring the substrate.

Method for measuring the concentration or change in concentration of a redox-active substance and corresponding device

Especially in order to carry out the so-called enzyme-coupled DNA hybridization test in a closed cartridge including a microfluid system, using stored dry reagents, the reagents must be dissolved in the microfluid system and transported into the measuring chamber directly before the measurement. During the dissolution of the reagents in water, air cushions that cannot reach the measuring chamber must absolutely be prevented from forming upstream of the reagent liquid. According to an embodiment of the invention, the liquid measuring sample and the liquid reagents are transported in such a way that the air cushion is directed into the waste line and the measuring sample and the reagents are then introduced into the measuring chamber without any air bubbles. In this way, measuring errors can be avoided.

Method for carrying out an electrochemical measurement on a liquid measuring sample in a measuring chamber that can be accessed by lines, and corresponding arrangement

Electrically charged molecules need to be transported in order to create a DNA sensor. The following measures are undertaken: base metals are introduced into a solution as a positive ion; negatively charged molecules are transported in an opposite direction and are enriched in the vicinity of the measuring electrodes. Binding-specific separation of the charged molecules can be achieved by forming metal layers on the measuring electrodes by depositing metal ions from the solution when a suitable potential is selected. Target DNA can more particularly be introduced into the vicinity of the catcher molecules on the measuring electrodes and non-specifically bound DNA can be removed. According to the associated device, the electrode arrangement may be associated with a sacrificial electrode made of more base metal than the material of the measuring electrodes. The measuring electrodes in particular may be made of noble metal, preferably gold, and the sacrificial electrode may be made of copper.

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Nürnberg, Germany

Heike Barlag