Glykos Finland Oy

Heidi Salminen

Jari Natunen

Markku Saloheimo

Anne Kanerva

Juhani Saarinen

Ann Westerholm-Parvinen

Anne Huuskonen

Christopher Landowski

Jukka Hiltunen

Bernhard Helk

Annamari Heiskanen

Christian Ostermeier

Benjamin Patrick Sommer

Ramon Wahl

Anne Leppänen

Georg Schmidt

Eero Mustalahti

The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such ascells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing &#x3b1;1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.

Production of glycoproteins with mammalian-like N-glycans in filamentous fungi

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Espoo, Finland

Heidi Salminen