Wisconsin Alumni Research Foundation

Heidi Flewelling Kaeppler

Michael William Petersen

Brian J Martinell

Shawn Michael Kaeppler

Edward James Williams

Frank McFarland

Robert Wayne Harnish

Ray Collier

Amy Jo Miyamoto

Nathaniel Schleif

Frank Mcfarland

The present invention provides pollen compositions, methods and kits for making said compositions, and methods of using said compositions to fertilize a plant. The pollen within these compositions has increased longevity and can be used to fertilize plants for an extended period of time as compared to pollen prepared using conventional methods. Also provided are methods of collecting pollen from a maize plant by maturing individual tassel branches off of the plant and vessels for collecting the pollen from these individual branches.

Pollen collection methods, pollen compositions and uses thereof

Many plants are recalcitrant to available plant transformation methods, which hinders the development of genetically modified plants. To address this problem, the present invention provides constructs comprising a WUSCHEL-like homeobox 2a (WOX2A) gene from maize operably connected to a promoter. Methods of using these constructs to induce somatic embryogenesis in the tissues from a cereal monocot plants and plants produced using these methods are also provided.

Maize WOX2A over-expression induces somatic embryo formation

Disclosed herein are methods for sterile extraction, drying, storage, and transformation of immature and mature maize and monocot embryos. The present disclosure also describes a dried, storable, freezable value added maize and monocot explants.

Efficient monocot embryo extraction and preservation methods and novel uses thereof

A maize pollen composition containing polyether ether ketone and pollen for collecting pollen and storing pollen.

Maize pollen composition for collecting pollen and storing pollen

Disclosed herein are methods for the production ofmeristem explants from dry seeds. Also described are methods of transforming and gene editing using themeristem explants disclosed herein.

Methods of gene editing and transforming

Methods of gene editing and transforming cannabis

The present disclosure provides methods for the stable transformation of meristem explants from cowpeas () and dry beans ().

Agrobacterium-mediated and particle bombardment transformation method for cowpea and dry bean meristem explants

Disclosed herein are methods for the production of value added explants from the seeds of dicotyledonous cultivars of interest. Also described are methods of transformation using the value added explants produced by the methods disclosed herein.

Growing community of inventors

Oregon, WI, United States of America

Heidi Flewelling Kaeppler