Basf Plant Science Gmbh

Basf Plant Science Company Gmbh

Monsanto Technology LLC

Hee-Sook Song

Jeffrey A Brown

Hongmei Jia

Liqun Xing

John McMillan

Huihua Fu

Lesley Ireland

Paula Olhoft

Christian Dammann

Peifeng Ren

Luke Mankin

Kirk Francis

Marc Morra

Linda Patricia Loyall

Michael Kock

Allan R Wenck

Larry Nea

Dwight-Steven Hill

Effie Toren

John Mcmillan

Brendan S Hinchey

Haiping Hong

Ming Cheng

Leslie Grist

Libby Bernal

Sara Price

Diana Arias

Fang-Ming Lai

Timothy C Jensen

Yuwen Wang

Christina E Roche

Allan Richard Wenck

Alleson Dobson

The present invention is concerned with the provision of means and methods for gene expression. Specifically, it relates to a polynucleotide comprising an expression control sequence which allows for seed specific of a nucleic acid of interest being operatively linked thereto in plants. Furthermore, vectors, host cells, transgenic plants and methods for expressing nucleic acids of interest are provided which are based on the said polynucleotide.

Whole seed specific promoter

The present invention is in the field of genetics, especially plant genetics, and provides agents capable of controlling gene expression. The present invention specifically provides sequences of naturally occurring, tissue-specifically expressed microRNAs. The invention further provides for transgenic expression constructs comprising sequences encoding such microRNAs. By incorporation of the microRNA encoding sequence the expression from the expression construct is specifically silenced in the tissue where the naturally occurring microRNA is naturally expressed. Thereby the expression profile resulting from the promoter is modulated and leakiness is reduced. The invention further provides for a method for modulating transgenic expression by incorporating sequences encoding the microRNAs into transgenic expression constructs. The compositions and methods of the invention can be used to enhance performance of agricultural relevant crops and for therapy, prophylaxis, research and diagnostics in diseases and disorders, which afflict mammalian species.

Methods controlling gene expression

The present invention is in the field of genetics, especially plant genetics, and provides agents capable of controlling gene expression. More specifically the inventions relates to methods for engineering ta-siRNA primary transcripts in order to target gene-of-interest (GOI) and control their expression. The invention further provides for a method for modulating transgenic expression by said engineered ta-siRNAs.

Methods for controlling gene expression using ta-siRNA

The invention relates to efficient, high-throughput methods, systems, and DNA constructs for identification and isolation of transcription termination sequences. The invention relates further to specific terminator sequences identified by said methods isolated from rice.

Method for isolation of transcription termination sequences

The present invention is in the field of genetics, especially plant genetics, and provides agents capable of controlling gene expression. The present invention specifically provides sequences of naturally occurring, tissue-specifically expressed microRNAs. The invention further provides for transgenic expression constructs comprising sequences encoding said microRNAs. By incorporation of the microRNA encoding sequence the expression from said expression construct is specifically silenced in the tissue where the naturally occurring microRNA is naturally expressed. Thereby the expression profile resulting from the promoter is modulated and leakiness is reduced. The invention further provides for a method for modulating transgenic expression by incorporating sequences encoding said microRNAs into transgenic expression constructs. The compositions and methods of the invention can be used to enhance performance of agricultural relevant crops and for therapy, prophylaxis, research and diagnostics in diseases and disorders, which afflict mammalian species.

The present invention relates to an expression cassette for regulating seed-specific expression of a polynucleotide of interest, said expression cassette comprising a transcription regulating nucleotide sequence, to a vector comprising said expression cassette, host cells and transgenic plants comprising the expression cassette, and methods of producing said transgenic plants.

Expression cassettes for endosperm-specific expression in plants

The present invention relates to expression cassettes comprising at least one transcription regulating nucleotide sequence obtainable from the group of genes of monocotyle-donous plants consisting of caffeoyl-CoA-O-methyltransferase genes, C8,7-sterol isomerase genes, hydroxyproline-rich glycoprotein (HRGP) genes, lactate dehydrogenase genes, and chloroplast protein 12 like genes. More preferably the transcription regulating sequences are obtainable fromor. The transcription regulating sequences are especially useful for root/kernel-preferential, leaf/endosperm-preferential, root/silk/kernel-preferential, or constitutive expression.

Expression cassettes for regulation of expression in monocotyledonous plants

The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.

Expression enhancing intron sequences

The present invention relates 'disarmed' strain variants of Agrobacterium strain K599 (NCPPB 2659), 'disarmed' plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.

Disarmedstrains, ri-plasmids, and methods of transformation based thereon

The invention relates to efficient, high-throughput methods, systems, and DNA constructs for identification and isolation of transcription termination sequences. The invention relates further to specific terminator sequences identified by the methods isolated from rice.

The present invention relates to improved methods and means for transformation of soybean () based on a D-alanine and/or D-serine selection.

D-amino acid selection for soybean

The present invention relates to the field of agricultural biotechnology. Disclosed herein are expression constructs with expression specificity for the starchy endosperm and/or the germinating embryo, transgenic plants comprising such expression constructs, and methods of making and using such DNA constructs and transgenic plants.

Starchy-endosperm and/or germinating embryo-specific expression in mono-cotyledonous plants

The present invention relates 'disarmed' strain variants ofstrain K599 (NCPPB 2659), 'disarmed' plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.

Growing community of inventors

Raleigh, NC, United States of America

Hee-Sook Song