Cellink Bioprinting Ab

Cellink Ab

Hector Martinez

Erik Gatenholm

Erik Sternå

Jockum Svanberg

Paul Gatenholm

Bryan D Jones

Adam Micha

Anton Andrén

Ginger Lohman

Christen J Boyer

Ali Cakir

Anton Andren

Hany AbuShall

Kyung Hun Jung

Peter Faltpihl

Michela Schettino

Eric Bronnenkant

Patrick Thayer

Mateusz Piotrzkowski

A bioprinter () comprising at least one printhead () with nozzle (), a printbed () onto which the printhead () is arranged to print an ink, an ultrasonic sensor arrangement arranged at the printbed (), a controller () arranged to control movement of the printhead/nozzle () and to perform calibration of the printhead/nozzle () based on information from the ultrasonic sensor arrangement.

Bioprinter and method for calibration of the bioprinter

A material cartridge arrangement () for a dispensing system, comprising a material cartridge () having a first end (), an opposing second end () and an ink material channel () extending between the first and second ends in the material cartridge (), the ink material channel () being bounded by an ink material channel wall/walls () extending between the first () and second ends (). An ink material pressurising device () is arranged to cause ink material hold in the ink material channel () to flow in a direction from said first end () towards said second end () and through an ink material outlet. At least one temperature sensing element () is arranged at a position along the direction of extension of the ink material channel () and arranged to measure a temperature at the ink material channel wall ().

Material cartridge arrangement for a dispensing system

Systems and methods for optical assessments of bioink printability are described. The systems and methods include the use of hardware, software and optical targets to aid in the evaluation of bioink printability. The optical targets are developed and fabricated from 3D printable materials determined to be 'nozzle fidelic' and/or materials that possess well characterized thermosensitivity. The optical targets make it possible to rapidly compare and evaluate bioink printability and can be easily customized and tailored for specific applications.

Systems and methods for optical assessments of bioink printability

A three-dimensional (3D) bioprinting method and system are disclosed. The method includes disposing/immersing a printing platform or surface into a first bioink, such as a bioink resin, curing one or more layer of the first bioink resin onto the printing platform or surface, and removing the printing platform or surface from the first bioink resin. The process is repeated with a second bioink resin such that the second bioink resin is cured on top of the one or more layer of first bioink resin, and can be further repeated with a third or even fourth bioink resin. By varying constituents of one or more or each bioink resin (such as living cell type or polymer), complex, multilayered tissues can be engineered. A system capable of performing the method is also disclosed.

Temperature-controlled multi-material overprinting

The present invention relates to preparation of bioink composed of cellulose nanofibril hydrogel with native or synthetic Calcium containing particles. The concentration of the calcium containing particles can be between 1% and 40% w/v. Such bioink can be 3D Bioprinted with or without human or animal cells. Coaxial needle can be used where cellulose nanofibril hydrogel filled with Calcium particles can be used as shell and another hydrogel based bioink mixed with cells can be used as core or opposite. Such 3D Bioprinted constructs exhibit high porosity due to shear thinning properties of cellulose nanofibrils which provides excellent printing fidelity. They also have excellent mechanical properties and are easily handled as large constructs for patient-specific bone cavities which need to be repaired. The porosity promotes vascularization which is crucial for oxygen and nutrient supply. The porosity also makes it possible for further recruitment of cells which accelerate bone healing process. Calcium containing particles can be isolated from autologous bone, allogenic bone or xenogeneic bone but can be also isolated from minerals or be prepared by synthesis. Preferable Calcium containing particles consist of &#x3b2;-tricalcium phosphate which is resorbable or natural bone powder, preferably of human or porcine origin. The particles described in the present invention have particle size smaller than 400 microns, or more preferably smaller than 200 microns, to make it possible to handle in printing nozzle without clogging and to obtain a good resolution. Cellulose nanofibrils can be produced by bacteria orbe isolated from plants. They can be neutral, charged or oxidized to be biodegradable. The bioink can be additionally supplemented by other biopolymers which provide crosslinking. Such biopolymers can be alginates, chitosans, modified hyaluronic acid or modified collagen derived biopolymers.

Preparation and applications of 3D bioprinting bioinks for repair of bone defects, based on cellulose nanofibrils hydrogels with natural or synthetic calcium phosphate particles

The present disclosure relates to a 3D bioprinter () comprising a base unit (). The base unit () has a support () adapted for mounting of at least one toolhead (), a communication interface part () for communication of data with the at least one toolhead (), when mounted, and a base unit processing element () adapted to communicate with a toolhead processing element () of the at least one toolhead over said communication interface part (). The present disclosure relates further to a 3D bioprinter toolhead. The present disclosure relates further to a method for bioprinting a construct.

3D bioprinters, a 3D bioprinting toolhead and a method for 3D bioprinting a construct

The present disclosure relates to a 3D printer () for 3D printing of a construct. The 3D printer () has a print bed (). The 3D printer further comprises at least one actuating tool head () with an extrusion element (), wherein the extrusion element and the print bed are movable in relation to each other. The 3D printer also comprises at least one sensor () arranged to sense a force applied to the print bed () by the extrusion element (), or vice versa. The 3D printer additionally comprises a control element () arranged to detect when the sensed force exceeds a predetermined value and to record a position of the print bed or extrusion element related to the detection that the predetermined value is exceeded. The present disclosure also relates to corresponding methods and computer programs.

3D printer and a method for 3D printing of a construct

The present invention relates to a double network bioink that is customizable and tailorable in terms of mechanical characteristics, diffusivity, and biological functionality that is printable at room temperature and cytocompatible. Double network bioinks of the present invention can be utilized as a standard for comparison and evaluation of bioinks and during calibration and/or are suited for the education field as a 'blank slate' bioink that can be utilized to convey different scientific concepts. Specific bioinks included comprise: one or more biocompatible or non-biocompatible thickener; one or more polyethylene glycol based crosslinkable network; one or more photoinitiator; and/or optionally, one or more additives to impart desired and/or different characteristics to the bioink. In embodiments the thickener and polyethylene glycol based crosslinkable network form a structure comprising two interpenetrating networks.

Double network bioinks

The present disclosure relates to a print bed () for regulating a temperature of the print bed (). The print bed () comprises at least one Peltier element (), each Peltier element having opposite first and a second surfaces (). The print bed () further comprises at least one heatsink (). The at least one Peltier element () is arranged to have each respective first surface () facing a print surface () of the print bed (). The at least one heatsink () is thermally connected to the Peltier element () and arranged to transfer heat generated by the at least one Peltier element () and dissipate the transferred heat away from the at least one Peltier element (). The present disclosure further relates to corresponding 3D-printers, methods, computer programs and modules.

Growing community of inventors

Gothenburg, Sweden

Hector Martinez