Bio-Rad Laboratories, Inc.

Hector Juarez-Salinas

Gary S Ott

Robert Bruce Wallace

Luis A Ugozzoli

Sheldon C Engelhorn

Gary Steven Ott

Sheldon Craig Engelhorn

This invention provides methods and compounds relating to the use of deoxyribose nicotinamide adenine dinucleotide (dNAD.sup.+) analogues in NAD.sup.+ -dependent ligation reactions. The compounds and methods may be used in NAD.sup.+ -dependent ligation reactions generally, and to increase the specificity of NAD.sup.+ -dependent ligation reactions.

Use of deoxyribose nicotinamide adenine dinucleotide to enhance the

A novel method is disclosed for the purification of monoclonal antibodies from antibodies of the same isotype but different light chain composition which are frequently present in the fluids in which such antibodies are generated. According to the method, the mixture is placed in a hydroxylapatite chromatography column and eluted with a buffer solution in a concentration gradient elution. With the appropriate optimization of system parameters, a separation of antibodies effective for both analytical and preparative purposes is obtained.

Use of hydroxylapatite for the analysis and preparation of purified

Unusually strong binding of IgG to protein A is achieved by contacting these components in the presence of a medium containing a high concentration of salt. Although such binding is of general utility, a particularly useful application is the purification of monoclonal antibodies from ascites fluid by affinity chromatography.

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Larkspur, CA, United States of America

Hector Juarez-Salinas