Illumina Corporation

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Hayden Black

Brian Douglas Mather

Hai Quang Tran

Alexandre Richez

Lewis J Kraft

Sean M Ramirez

Xavier Von Hatten

Ludovic Vincent

Hong Xie

Julia Morrison

Gianluca Andrea Artioli

Wei Wei

Edwin Li

Maxwell Zimmerley

Krystal Sly

Innsu Daniel Kim

Ryan Sanford

Sojeong Moon

In one example, a flow cell includes a substrate, an electrode positioned on the substrate, and a patterned material positioned on the electrode. In this example, the patterned material includes depressions separated by interstitial regions, and a functionalized surface of the electrode is exposed at each of the depressions. In this example, the flow cell further includes a primer grafted to the functionalized surface in each of the depressions. In another example, a flow cell includes a substrate and a patterned electrode positioned on the substrate. In this other example, the patterned electrode includes depressions separated by interstitial regions, and a functionalized surface of the substrate exposed at each of the depressions. In this other example, a primer is grafted to the functionalized surface in each of the depressions.

Flow cells and sequencing kits

An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

Protective surface coatings for flow cells

Provided in one example is a sensor having at least one fissure, the fissure being at least partially filled by at least one polymer formation extending vertically within a passivation layer. The polymer formation protects the underlying metal containing layer from corrosive solutions. Provided in another example is a method of forming the polymer formation in a fissure of a sensor.

Passivating fissures in substrates

Growing community of inventors

San Diego, CA, United States of America

Hayden Black