Fred Hutchinson Cancer Center

Fred Hutchinson Cancer Research Center

Hatice Seda Kaya Okur

Steven Henikoff

Peter James Skene

Terri Dawn Bryson

A method for detecting the binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in a cell, including: contacting a permeabilized cell or nucleus with a specific binding agent that specifically recognizes the chromatin-associated factor of interest, wherein the specific binding agent is linked to a nuclease that is inactive or an activatable transposome; activating the nuclease or transposase, thereby excising the sequence of chromatin DNA bound to the chromatin-associated factor of interest; isolating the excised DNA; and determining the sequence of the excised DNA, thereby detecting binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in the cell.

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Redmond, WA, United States of America

Hatice Seda Kaya Okur