Lumigen, Inc.

Beckman Coulter, Inc.

Ahuratech LLC

Wayne State University

Other

Tropix, Inc.

Bayer Corporation

Hashem Akhavan-Tafti

Zahra Arghavani

Renuka DeSilva

Arthur P Schaap

Ravinder K Gupta

Renuka De Silva

Wenhua Xie

Robert A Eickholt

Richard S Handley

Kumar Thakur

Barry A Schoenfelner

Kenneth S Lauwers

Guoping Wang

Mark D Sandison

Michael Salvati

James D Mendoza

Bruce H Odegaard

Nir Shapir

Terri McLernon

Ying Chen

Dean G Binger

Ali Ghiaseddin

Lakshmi Sireesha Kaanumalle

A Paul Schaap

Katsuaki Sugioka

Randall K Larkin

Mojtaba Akhavan-Tafti

David Jorge Schiffrin

Robert Wilson

Ehsan Yakhshi-Tafti

Robert F Adolfsen

Lekkala V Reddy

Yumiko Sugioka

Khaledur S Rashid

Ali Bolandi

James Mendoza

Dean Binger

Bruce Odegaard

Lakshmi S Kaanumalle

Richard Handley

Terri Mclernon

Methods and devices for the characterization of biological specimens by the use of electroluminescent materials. When placed in close proximity or direct contact to a biological specimen and an electrical signal is transmitted, electroluminescence is generated in response to the presence of the specimen. The electroluminescence produced can be in the form of an image of the specimen. The image is captured by an optical device that collects light and displays or otherwise processes the image according to the particular need or purpose. In general, the method requires preparing an electroluminescent assembly including the biological specimen, a dielectric layer, and a substrate, put together in any order. The method uses an electrical signal transmitted to the assembly. The device may be configured in a closed-circuit electrical configuration or it may be in a configuration where the EL assembly is at open circuit with respect to the power source.

Electroluminescent methods and devices for characterization of biological specimens

Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.

Solution phase homogeneous assays

Methods of producing luminescence by application of a time-varying electrical signal to an electroluminescent device are disclosed whereby the entire system remains at open circuit. At least one article, substance or material, the 'object', is employed to alter the electrical signal to the area of the electroluminescent device to a level sufficient to change light emission. Methods are disclosed to relate the light intensity thus produced to a property of the object thereby allowing a measurement of the property. The method may optionally use one or more additional circuit components.

Electroluminescent methods and system for real-time measurements of physical properties

Methods of producing luminescence by application of a time-varying electrical signal by a single wire or transmission medium to an electroluminescent device are disclosed whereby the entire system remains at open circuit. At least one substance, material or object is employed to enhance an electric field in the area of the electroluminescent device to a level sufficient to cause light emission. Methods are disclosed to relate the light intensity thus produced to a property of the electric field-enhancing substance, material or object thereby allowing a measurement of the property. The electric field-enhancing substance, material or object(s) can comprise part of the transmission medium or be placed on or near the electroluminescent device.

Open-circuit electroluminescence

A method is presented for storage and on-demand release of nanoparticles. Nanoparticles produced by this method can be dried and stored for an extended period of time and subsequently released on-demand in a solvent of choice to form stable suspensions without the need for additional surfactants or stabilizers and without any loss in functionality or material properties. This method can be used to store various categories of nanomaterials including metals, metal oxides, metal chalcogenides, magnetic, polymeric and semiconductor nanoparticles.

Method for storing and releasing nanoparticles

Methods of producing light in liquid media are provided using nanoparticles capable of generating electroluminescence when stimulated by an electrical signal. The nanoparticles are provided as a label on a target species or on a specific binding partner of the target species to be detected in a test method. The nanoparticle-labeled species are drawn into operable proximity to electrodes which, when energized by a power source, excite the nanoparticles to produce electroluminescence. Methods of performing binding assays are described using the disclosed methods.

Electroluminescent binding assays

A method is presented for producing light using a liquid media. The method includes: hosting nanoparticles in a liquid media; disposing a pair of electrodes in direct contact with the liquid media; and generating an excitation signal between the electrodes in the pair of electrodes, thereby illuminating a portion of the nanoparticles.

System and method for producing light in a liquid media

A method is presented for synthesizing particles in the presence of a solid phase. Of note, sorption is used to associate a precursor(s) for synthesizing the particles onto or into the surface of a host structure prior to the chemical reaction that results in the particles being formed in or on surface of the host structure. Particles produced by this method can be stored for long durations and released on-demand in a solvent of choice to form stable suspensions without the need for any additional surfactants or stabilizers.

Method for synthesizing particles in the presence of a solid phase

A method is presented for producing light using a liquid media. The method includes: suspending nanoparticles in a polar liquid media; disposing a pair of electrodes in direct contact with the liquid media; and generating an excitation signal between the electrodes in the pair of electrodes, thereby illuminating a portion of the nanoparticles.

The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.

Fluorescent dyes and uses thereof

Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.

Methods using novel chemiluminescent labels

Growing community of inventors

Howell, MI, United States of America

Hashem Akhavan-Tafti