Shiseido Company, Limited

Haruyo Yamanishi

Tsutomu Soma

Yuzo Yoshida

Yumiko Ishimatsu

Masato Iino

The present disclosure relates to a serum-free medium for culturing of DS cells containing platelet derived growth factor (PDGF), or to a method for culturing of dermal sheath (DS) cells, using serum-free medium comprising PDGF.

Serum-free medium containing PDGF for DS cells

An agent comprising one or more components selected from among cowberry derivatives, mangosteen derivatives,root derivatives, inositol and inositol phosphate, as an active ingredient, is a novel agent that can effectively promote production of platelet-derived growth factor-BB (PDGF-BB), which contributes to production of mesenchymal stem cells and stabilization of stem cells.

Platelet-derived growth factor-BB production promotor, and mesenchymal stem cell production accelerator, stem cell stabilizer and dermal regenerator comprising the same

This method, which screens drugs that activate the skin, is characterized by causing candidate drugs to act on vascular endothelial cells, and selecting drugs that enhance the expression of PDGF-BB by the cells as a skin activation agent.

Skin activation by acceleration of PDGF-BB activity

The invention provides a method of isolating dermal stem cells, having the steps of subjecting cells separated from the skin by enzyme treatment to suspension culture, and isolating cells positive for stem cell markers from the cultured cells.

Method of isolating dermal stem cells

An object of the present invention is to promote hair growth or hair regeneration by promoting formation and/or regeneration of hair follicles. A method is provided for promoting formation and/or regeneration of hair follicles, comprising maintaining or increasing expression of one or a plurality of genes involved in cell adhesion in dermal papilla cells.

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Yokohama, Japan

Haruyo Yamanishi