Suntory Limited

Suntory Limited of 1-40, Dojimahama

Haruyo Hatanaka

Yuji Shibano

Sakayu Shimizu

Teruo Amachi

Toshihiko Ashikari

Yoshikazu Tanaka

Jun Ogawa

Toru Nakayama

Yoshiki Tani

Yasuyoshi Sakai

Motoo Sumida

Yoshihide Suwa

Hideko Yomo

Hirofumi Koda

Hiroto Kondo

Takehiro Matsumoto

Novel purine nucleosidase derived from Ochrobactrum anthropi microorganisms, a gene system coding therefor, uses therefor and particularly its use in the production of beer.

Purine nucleosidase

Process for manufacturing beer

The present invention relates to an expression vector which is induced by methanol and/or glycerol utilizing an alcohol oxidase gene of a methylotrophic yeast; a recombinant methylotrophic yeast containing said vector and capable of accumulating a significantly high amount of an expression product of a heterologous gene; and a method for producing useful products utilizing said recombinant. The expression vector is constructed by utilizing the promoter and terminator of an alcohol oxidase gene of methylotrophic yeast. Further, a significantly high amount of adenylate kinase, cytochrome C552 or peroxidase is produced by using said expression vector.

Vector having promoter that is inducible by methanol and/or glycerol

A peroxidase gene derived from Arthromyse ramosus and its cloning and expression in E. coli and yeast is disclosed. The peroxidase encoded by this gene is not-specific and does not contain isozymes and hence is suitable as a diagnostic reagent or as a marker enzyme in enzyme innunoassays.

Peroxidase gene of microbial origin

Growing community of inventors

Kyoto, Japan

Haruyo Hatanaka