Ajinomoto Co., Ltd.

Haruo Heima

Takeshi Nagai

Satoshi Ueda

Hiroyuki Sato

Makoto Ozawa

Tsuyoshi Nakamatsu

Takashi Iizuka

Hiroshi Matsui

Keiichi Yokoyama

Yoshimi Kikuchi

Masayo Date

Yukiko Umezawa

The object of the present invention is to provide a method of producing a heterologous protein by making a coryneform bacterium to produce and efficiently extracellularly secrete (secreto-production) an industrially useful heterologous protein. According to the present invention, a genetic construct is used where a gene sequence encoding an intended protein which is ligated to the downstream of a sequence encoding the signal peptide derived from a coryneform bacterium, the gene construct is introduced into a mutant coryneform bacterium which has a capacity of secreting the heterologous protein at least 2-fold higher than the wild typeATCC 13869, the mutant coryneform bacterium is cultured and the extracellularly released heterologous protein is recovered.

Methods for secretory production of proteins

The present invention provides a feed additive composition for ruminants containing a biologically active substance therein. When administered to ruminants, the composition containing the active substance is stably protected in the rumen while efficient release of the active substance is realized in the abomasum and the following digestive tract. Thus, the released active substance is effectively digested and absorbed by ruminants.

Feed additive composition for ruminants

A feed additive composition is provided having a core which contains a biologically active substance and which is coated with a coating composition which contains components a) and b): a) at least one substance selected from the group consisting of linear or branched saturated or unsaturated aliphatic monocarboxylic acids of 12-22 carbon atoms or salts thereof, hardened vegetable fats and oils, hardened animal fats and oils, and wax; and b) at least one of substances i) and ii):

The present invention relates to a biologically active substance that is stable in the rumen of ruminants and released in the abomasum and its subsequent digestive tract. The biologically active substance has the additional properties of being digested and absorbed with good efficiency.

Feed additive for ruminants

Growing community of inventors

Kawasaki, Japan

Haruo Heima