Takara Shuzo Co., Inc.

Takara Bio Inc.

Harumi Ueno

Ikunoshin Kato

Hiroaki Sagawa

Jun Tomono

Takeshi Sakai

Atsushi Oshima

Yoshizumi Ishino

Kaori Akiyama

Masayuni Kishimoto

A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.

Sulfated fucogalactan digesting enzyme gene

A vector having a region encoding a cold shock protein gene mRNA-origin 5'-nontranslated region, characterized in that the 5&#x2032;-nontranslated region has a mutation having been transferred therein so as to change the distance of the stem structure formed by the region.

Cold-induced expression vector

A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease-gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.

Gene isolation method

Therapeutic or preventive agents for diseases due to the action of aldose reductase; and aldose reductase inhibitors, characterized by containing at least one compound having an aldose reductase inhibiting activity which is selected from among 2,5-dihydroxytetra-hydro-2-furancarboxylic acid, derivatives of the same, optical isomers of both and pharmacologically acceptable salts of them.

Therapeutic agents

Plasmid

An isolated BalI restriction enzyme gene and an BalI isolated modification enzyme gene are disclosed. A process for preparing a large amount of BalI restriction enzyme which comprises culturing a transformant containing said genes and collecting BalI restriction enzyme from the culture broth is also disclosed.

Growing community of inventors

Shiga, Japan

Harumi Ueno