Other

Canon Kabushiki Kaisha

Shiseido Company, Limited

Sekisui Chemical Co., Ltd.

Nippon Zeon Company, Ltd.

Keio University

Haruma Kawaguchi

Hiroshi Handa

Takeshi Miyazaki

Akira Asai

Yuichiro Mori

Takashi Oka

Izumi Omoto

Takayuki Oka

Katsuhiko Yagi

Yasuji Ohtsuka

Wataru Wakui

Akiko Sudo

The present invention provides a film composition effective for correcting irregularities on the skin surface, which has stretchability and flexibility as a result of crosslinkable protein microparticles in the film composition being crosslinked by transglutaminase, as well as superior air permeability and moisture evaporation properties as a result of forming a film with the crosslinkable protein microparticles.

Transglutaminase crosslinked protein microparticle film composition

It is an object of the present invention to provide magnetic substance-encapsulated particles which have uniform magnetism, high dispersion stability and a narrow particle size distribution, a method of producing the same, particles for immunoassay formed by using the magnetic substance-encapsulated particles and a method of immunoassay in which the magnetic substance-encapsulated particles or the particles for immunoassay are used. The present invention relates to a magnetic substance-encapsulated particle, which comprises an organic polymer material and a magnetic substance having an average particle size of 1 to 30 nm, the magnetic substance being contained within a particle in a state of being dispersed.

Particle having magnetic material incorporated therein, process for producing the same, particle for immunoassay and method of immunoassay

A compound possessing physiological activity is coupled to a styrene-glycidyl methacrylate polymer through a spacer. Compounds that may be used include receptors such as proteins, and 3-&lsqb;(5-(2,3-dimethoxy-6-methyl-benzoquinonyl)&rsqb;-2-nonyl-2-propionic a preferred spacer is an ethylene glycol diglycidyl ether derivative. Preferably, the whole surface of the styrene-glycidyl methacrylate polymer in microsphere form is covered with glycidyl methacrylate. The microsphere may be used for isolating and detecting substances such as proteins that bind to the coupled compound.

Method of screening substances with a glycidyl methacrylate covered styrene-glycidyl methacrylate polymer

A microsphere is prepared containing a compound possessing physiological activity coupled to a styrene-glycidyl methacrylate polymer through a spacer. Compounds that may be used include receptors such as proteins, and 3-&lsqb;(5-(2,3-dimethoxy-6-methyl-benzoquinonyl)&rsqb;-2-nonyl-2-propionic acid. A preferred spacer is an ethylene glycol diglycidyl ether derivative. Preferably, the whole surface of the styrene-glycidyl methacrylate polymer is covered with glycidyl methacrylate. The microsphere may be used for isolating and detecting substances such as proteins that bind to the coupled compound.

Microsphere containing glycidyl methacrylate covered polymer and spacer coupled compound for isolating substances

A substance possessing physiological activity is coupled to a styrene-glycidyl methacrylate polymer through a spacer, and is used to isolate from a mixture a substance that can adhere to the substance possessing physiological activity. Preferably the polymer is in the form of a microsphere, the substance possessing physiological activity is 3-[(5-(2,3-dimethoxy-6-methyl-1,4-benzoquinonyl)]-2-nonyl-2-propionic acid or derivative thereof, and the spacer is an ethylene glycol diglycidyl ether derivative. The mixture may be a cell extract and the substance isolated a protein that is a receptor to the substance possessing physiological activity.

Isolating substances with

A dry detection reagent for detecting an immunologically active substance is prepared containing solid fine polymer particles immobilizing a substance immunologically active for the substance detected. The polymer particles are prepared by one of the following methods: a) ternary copolymerization of a monomer having carboxyl groups, a monomer having amino groups and styrene or its derivatives; b) binary copolymerization of a monomer having carboxyl groups and styrene or its derivatives, and reacting part of the carboxyl groups with a bifunctional amine such as ethylene diamine to provide amino groups; or c) copolymerizing styrene or its derivatives with a monomer having amide groups, and converting part of the amide groups to amino groups and carboxyl groups with the Hofmann reaction, or hydrolyzing part of the amide groups to form carboxyl groups. Preferably, styrene and acrylamide having amide groups are copolymerized and part of the amide groups are hydrolyzed to carboxyl groups or part of the amide groups are converted to carboxyl groups and amino groups by the Hofmann reaction. The immunologically active substance used for detecting is immobilized on the particles and the particles are dried to produce the dry reagent. To carry out detection, the dry reagent is dispersed in a liquid, the liquid is mixed with a sample containing the substance to be detected which becomes bound to the substance immobilized on the particles and the bound substance is optically detected. The dry reagent is stable during storage and has excellent re-dispersibility in a liquid.

Dry detection reagent containing acrylamide/styrene copolymer particles

There are disclosed a DNA-immobilized microsphere comprising DNA chains having base sequences which bind a specific protein specifically, and a carrier having a particle size of not more than 50 .mu.m and not less than 0.01 .mu.m which does not adsorb any protein, said carrier and said DNA chains being bound to each other by a chemical bond, and a process for purifying a protein using said microsphere.

Growing community of inventors

Yokohama, Japan

Haruma Kawaguchi