Matsushita Electric Industrial Co., Ltd.

Kyoto Daiichi Kagaku Co., Ltd.

Haruhiro Tsutsumi

Shiro Nankai

Toshihiko Yoshioka

Yoshinobu Tokuno

Hideyuki Baba

Shin Ikeda

Mariko Kawaguri

Shoji Miyazaki

Syoji Miyazaki

Eriko Yamanishi

Takatoshi Uchigaki

Hiroshi Hyodo

Masahiro Yoshioka

Naoki Tanimoto

Kyozo Terao

Minoru Fukuda

A biosensor having an electrode layer, which includes a working electrode and a counter electrode, and a reagent layer are formed on an insulating support. Further, a spacer having a long and narrow cut-out portion on the reagent layer is bonded to a cover having an air hole to form a cavity that sucks blood as a liquid sample by capillary phenomenon, and a portion of side walls of the spacer and the cover, which side walls face the cavity, is subjected to a treatment for making the portion itself have hydrophilicity. Accordingly, when blood is sucked into the cavity by capillary phenomenon, the suction is promoted, and the performance of a sensor of the biosensor is improved. Further, the process of manufacturing the sensor is simplified, thereby resulting in increased productivity.

Biosensor

The biosensor of this invention can quantify a substrate in a sample liquid by electrochemically measuring the amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase. The biosensor has an electrically insulating substrate and an electrode system formed on the substrate including a working electrode, a counter electrode and a third electrode used for detecting a liquid junction. The third electrode can be used merely for detecting a liquid junction, or can be used as both a reference electrode and a liquid junction detecting electrode.

Biosensor, and a method and a device for quantifying a substrate in a

A biosensor for quantifying a specific compound has a reaction layer containing at least an enzyme, and an electrode system having a working electrode and a counter electrode formed on an insulating base plate; the biosensor detects the specific compound contained in a sample on the basis of an electrochemical response. In quantifying, the working electrode and the counter electrode are short-circuited before the voltage is applied therebetween. The short-circuiting eliminates measuring errors that may occur due to nonuniform dissolution of the reaction layer in a sample, and achieves highly reliable quantification of a specific compound.

Method for quantifying specific compound

A biosensor of the present invention is provided with an insulating base plate, an electrode system mainly consisting of a working electrode and a counter electrode formed on the insulating base plate, and a reaction layer on the electrode system. The counter electrode is partially a circular arc. A manufacturing method of the biosensor of the present invention is comprised of a step to form a base by arranging leads, an electrode system, and an insulating layer on an insulating base plate, and a step to form a reaction layer mainly composed of an enzyme on the electrode system. Since the counter electrode is partially in the shape of a circular arc, the reaction layer is easily formed and prevented from delaminating, whereby the measuring accuracy, preservative properties and reliability of the biosensor are improved. The present invention enables highly efficient biosensors to be mass produced at low cost.

The subject invention provides a simple sensor and an easy to handle biosensor measuring apparatus for the purpose of making measurements easily, rapidly and with high accuracy for specific components within a biological specimen such as blood. The biosensor of the subject invention is provided with a protrusion or a depression in a portion of a base having at least a measurement electrode and an opposing electrode. Also, the biosensor measuring apparatus is so configurated to have a mating means in the main body of the measuring apparatus freely supporting this biosensor to contact the said sensor's protrusion or depression, and further having the activating switch of the driving power supply located in this mating means. By means of these, it is possible to prevent wasteful measuring operations, as noted in the prior art biosensors, such as inserting the sensor backwards and making measurements with the sensor inserted backwards.

Biosensor and measuring apparatus using the same

A sensor 13 is inserted into a connector 14. A constant voltage required to obtain a response current is applied across the connector 14 by a voltage applying source 15 at timings required. A response current of the sensor 13 inserted into the connector 14 is converted into a voltage by a current-to-voltage converter 16, and the amount thereof is determined by a microcomputer, the analysis results being displayed onto a display unit.

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Ehime, Japan

Haruhiro Tsutsumi