Sumitomo Rubber Industries Limited

Tohoku University

Kanazawa University

Osaka University

Haruhiko Yamaguchi

Yukino Inoue

Kazuhisa Fushihara

Toru Nakayama

Seiji Takahashi

Satoshi Yamashita

Satoshi Kuroda

Teruhiko Terakawa

Tsubasa Yano

Keiji Takagi

Yuzuru Tozawa

Keiko Suzuki

Toshiya Muranaka

Yuko Sakurai

Chiharu Tani

Kanako Kobayashi

Provided is a method for producing a polyisoprenoid, which makes it possible to synthesize in vitro a polyisoprenoid having an unprecedented structure, such as a 100% cis-polyisoprenoid or a polyisoprenoid containing an allylic diphosphate derivative as an initiating terminal. Also provided is a method for producing a polyisoprenoid in vitro, which employs a gene coding for a neryl diphosphate synthase and rubber particles bound to a protein encoded by the gene, or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a gene coding for a neryl diphosphate synthase is linked to a promoter having a promoter activity that drives laticifer-specific gene expression to express a protein encoded by the gene specifically in laticifers.

Method for producing polyisoprenoid, vector, transformed plant, method for producing pneumatic tire, and method for producing rubber product

The present invention aims to provide a method for producing a trans-polyisoprenoid which can increase trans rubber production. The present invention is directed to a method for producing a trans-polyisoprenoid in vitro, which involves the use of a gene coding for a trans-prenyltransferase (tPT) family protein and further involves the use of rubber particles bound to a protein encoded by the gene, or a method for producing a trans-polyisoprenoid, which includes introducing into a plant a vector including a promotor having a promoter activity that drives laticifer-specific gene expression and a gene coding for a tPT family protein linked to the promotor to express a protein encoded by the gene specifically in laticifers.

Method for producing trans-polyisoprenoid, vector, transgenic plant, method for producing pneumatic tire and method for producing rubber product

Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.

Method for producing polyisoprenoid, transformed plant, method for producing pneumatic tire and method for producing rubber product

The present invention provides a method which can replacetechniques and which can efficiently produce transformed cells of a rubber-producing plant. The present invention relates to a method of producing transformed cells of a rubber-producing plant, which includes steps a) to e).

Method of producing transformed cells of rubber-producing plant

Provided is a method that can efficiently produce a transgenicplant in a short period. A method of producing a transgenicplant, including: an infection step of infecting a tissue fragment from aplant with ancontaining a plasmid containing a target gene or fragment thereof and hygromycin-resistance gene; a selective culture step of selecting the tissue fragment that has acquired the target gene from the tissue fragment obtained in the infection step by using hygromycin; a callus-inducing step of culturing the tissue fragment obtained in the selective culture step in a callus-inducing medium to form callus; a regeneration-inducing step of culturing the callus obtained in the callus-inducing step in a regeneration-inducing medium to form an adventitious embryo, an adventitious bud, and a shoot; and a rooting step of culturing the shoot obtained in the regeneration-inducing step in a rooting medium to root the shoot.

Method of producing transgenicplant

The present invention provides a method for producing a polyisoprenoid with which it is possible to enhance the rubber synthesis activity of rubber particles to increase natural rubber production. The present invention relates to a method for producing a polyisoprenoid in vitro, which involves the use of a gene coding for a cis-prenyltransferase (CPT) family protein and a gene coding for a Nogo-B receptor (NgBR) family protein, and further involves the use of rubber particles bound to proteins encoded by these genes; or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a promoter having a promoter activity that drives laticifer-specific gene expression is linked to a gene coding for a CPT family protein and a gene coding for a NgBR family protein, to express proteins encoded by the genes specifically in laticifers.

Production method for polyisoprenoid, vector, transgenic plant, production method for pneumatic tire, and production method for rubber product

The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.

Production method for rubber particles having membrane-binding protein bound thereto, production method for pneumatic tire, and production method for rubber product

A method for cultivating plants of the Asteraceae family, which promotes flower bud formation in the reproductive phase. A method for cultivating plants of the Asteraceae family, wherein the cultivation temperature in the reproductive phase is set lower by at least 5&#xb0; C. than the cultivation temperature in the vegetative phase to promote flower bud formation in the reproductive phase.

Method for cultivating plants of Asteraceae family

The present invention provides methods for enhancing the polyisoprenoid biosynthesis pathway. The present invention further provides isoprenoid-producing plants having an enhanced polyisoprenoid biosynthesis pathway, and methods for producing a polyisoprenoid using such an isoprenoid-producing plant. The present invention relates to methods for regulating the expression of specific protein(s) by a specific transcription factor; isoprenoid-producing plants into which has been introduced a gene encoding a specific transcription factor; and methods for producing a polyisoprenoid using such an isoprenoid-producing plant.

Method for modulating expression of specific protein using specific transcription factor, isoprenoid-producing plant having transgene encoding specific transcription factor, and method for producing polyisoprenoid using isoprenoid-producing plant

The present invention provides a method for extracting latex from a plant of the Asteraceae family and a method for cultivating a plant of the Asteraceae family, which allow such plants to be reused. The present invention relates to a method for extracting latex including a cutting step of cutting roots of a plant of the Asteraceae family in such a manner that the roots are partly left on the plant.

Method for extracting latex, method for cultivating plant of asteraceae family, method for producing pneumatic tire, and method for producing rubber product

The present invention provides a method enhancing the polyisoprenoid biosynthesis pathway. Also provided are an isoprenoid-producing plant having an overall enhanced pathway of polyisoprenoid biosynthesis, and a method of producing a polyisoprenoid using the isoprenoid-producing plant. The present invention relates to a method of regulating the expression of hydroxymethylglutaryl-CoA reductase by a bZIP transcription factor.

Method for adjusting expression of hydroxymethylglutaryl CoA reductase using bZIP-type transcription factor, isoprenoid-producing plant into which gene encoded for bZIP-type transcription factor is introduced, and method for manufacturing polyisoprenoid in which said plant is used

Provided is a method for producing rubber particles with a reduced coagulation tendency. The present invention relates to a method for producing rubber particles with a reduced coagulation tendency, the method including a step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a rubber elongation factor (REF) family protein to bind the REF family protein to the rubber particles.

Method for producing rubber particles with reduced coagulation tendency, method for producing pneumatic tire, and method for producing rubber product

The present invention provides a method for enhancing the overall pathway of polyisoprenoid biosynthesis. The present invention further provides an isoprenoid-producing plant having an overall enhanced pathway of polyisoprenoid biosynthesis, and a method of producing a polyisoprenoid using such an isoprenoid-producing plant. The present invention relates to a method of regulating by a cytokinin-responsive transcription factor the expression of at least one protein selected from the group consisting of hydroxymethylglutaryl-CoA reductase, isopentenyl diphosphate isomerase, cis-prenyltransferase, and small rubber particle protein.

Method for regulating expression of specific protein using cytokinin-responsive transcription factor, isoprenoid-producing plant having gene encoding cytokinin-responsive transcription factor introduced therein, and method for producing polyisoprenoid using said isoprenoid-producing plant

Growing community of inventors

Kobe, Japan

Haruhiko Yamaguchi