Kabushiki Kaisha Yakult Honsha

Harue Sone

Satoshi Matsubara

Fumiyasu Ishikawa

Tomoyuki Sako

Mayumi Kiwaki

Yasuhisa Shimakawa

Saeko Sawaki

Mitsuyoshi Kano

Hiroko Hayakawa

Mariko Kadota

Yukio Shirasawa

A bone metabolism improving agent characterized by containing as the active ingredient fermented soybean milk which is obtained by fermenting soybean milk with a lactic acid bacterium or a bifidobacterium. The bone metabolism improving agent is efficacious in treating and preventing diseases in association with lowered bone metabolism such as osteoporosis and, at the same time, satisfies requirements in cost and smooth intake.

Bone metabolism improving agent

Methods for transferring a foreign gene into a host chromosome by integrating the foreign gene into a vector by utilizing a lysogenic phage, for the purpose of deleting unnecessary genes derived from the vector, except for the foreign gene. One method comprises (a) preparing a vector with a lysogenic phage integration site (such as an attP site) arranged between a first partial sequence of the objective foreign gene to be transferred, but lacking one terminal region thereof and a second partial sequence of the gene, but lacking the other terminal region, the second partial sequence having an overlapping region with a portion of the first partial sequence; (b) integrating the vector obtained in step (a) into the host chromosome; and (c) screening from among the recombinants obtained in step (b) a recombinant from which unnecessary genes originating in the vector have been deleted owing to the homologous recombination mechanism functioning in the overlapping region between the first and second partial sequences.

Methods for transferring gene into chromosome

Growing community of inventors

Tokyo, Japan

Harue Sone