Basf Se Corporation

Evolva Sa

Hartwig Schroeder

Oskar Zelder

Holger Hartmann

Stefan Haefner

Birgit Hoff

Klaus Ditrich

Michael Breuer

Markus Pompejus

Christopher Koradin

Zheyuan Guo

Shakir Siraj Ratani

Manfred Ehresmann

Qingzhao Wang

Peter Oedman

Andreas Pletsch

Melanie Bonnekessel

Andrea Molt

Thomas Kaeding

Wolfgang Siegel

Michael Puhl

Karsten Koerber

Stephan Freyer

Martin Viertelhaus

Christoph Wittmann

Michael Naesby

Edzard Scholten

Franz Weber

Sebastian Wloch

Wolfgang Reichert

Joanna Martyna Krawczyk

Markus Schwab

Christophe Folly

Jacek Malisz

Curt Aimé Friis Nielsen

Burkhard Ernst

Stefanie Demming

Nico Boehmer

Anaelle Hatsch

Jakob Weber

Chung Huan Wong

Rüdiger Reingrubber

Ralf Hoock

Anne-Catrin Letzel

Daniel Seibert-Ludwig

Michael Barros Gross

Emiel Jan Kappert

Anna Christine Schroer

Karin Schein-Albrecht

Dirk Daegele

The present invention relates to a method for obtaining crystalline 2'-fucosyllactose from a 2&#x2032;-FL raw material, which contains 2&#x2032;-FL as a main constituent and at least 0.5% by weight, frequently at least 1% by weight, in particular at least 2% by weight, more particularly at least 5% by weight, and especially at least 8% by weight,based on the total amount of mono-and oligosaccharides in the raw material, of one or more mono- or oligosaccharides different from 2&#x2032;-FL, where the method comprises a)providing a solution of the 2&#x2032;-FL raw material in water, which does not contain more than 10% by weight, preferably not more than 7% by weight, more preferably not more than 5% by weight of organic solvents, based on the total amount of water; b) effecting the crystallization of 2&#x2032;-FL from the solution provided in step a) by inducing conditions of a controlled super saturation in the solution; and c) separating crystalline 2&#x2032;-FL from the mother liquor, and where during controlled supersaturation in step b) not more than 10% by weight, preferably not more than 7% by weight, more preferably not more than 5% by weight of organic solvents are present, based on the total amount of water present during step b).

Method for obtaining crystalline 2'-fucosyllactose

Described herein is a process of producing an organic compound, the process including: I) cultivating a genetically modified microorganism in a culture medium including sucrose as an assimilable carbon source to allow the genetically modified microorganism to produce the organic compound, and II) recovering the organic compound from the fermentation broth obtained in process step I) The genetically modified microorganism includes A) at least one genetic modification that leads to an increased activity of the enzyme encoded by the rbsK-gene, compared to the original microorganism that has not been genetically modified, and the original microorganism belongs to the family Pasteurellaceae. Also described herein are a genetically modified microorganism and the use thereof for the fermentative production of an organic compound from sucrose as an assimilable carbon source.

Process for producing an organic compound

Recombinant microorganism for improved production of fine chemicals The present invention relates to a recombinant nucleic acid molecule, a recombinant micro-organism, to a method for producing alanine and to the use of the recombinant nucleic acid molecule or the recombinant microorganism for the fermentative production of alanine.

Recombinant microorganism for improved production of fine chemicals

The present invention relates to a modified microorganism having, compared to its wildtype, &#x2014;a reduced activity of an enzyme encoded by the ptsA-gene, &#x2014;a reduced activity of an enzyme encoded by the ptsH-gene or &#x2014;a reduced activity of an enzyme encoded by the ptsA-gene and a reduced activity of an enzyme encoded by the ptsH-gene, wherein the wildtype from which the modified microorganism has been derived belongs to the family of Pasteurellaceae. The present invention also relates to a method for producing succinic acid and to the use of modified microorganisms.

Genetically modified microorganism for improved production of fine chemicals on sucrose

The present invention relates to a recombinant nucleic acid molecule, a recombinant microorganism, to a method for producing alanine and to the use of the recombinant nucleic acid molecule or the recombinant microorganism for the fermentative production of alanine.

The invention pertains to processes to produce dry biomass of pyripyropene producer organisms, processes to obtain pyripyropenes from such dry biomass, as well as to processes to produce compounds of Formula III and/or Formula IV and/or Formula V from the pyripyropenes obtained from the dry biomass. The invention does further pertain to the dry biomass itself, as well as processes using said dry biomass to obtain pyripyropenes for the production of compounds of Formula III and/or Formula IV and/or Formula V, including processes using said dry biomass to obtain pyripyropenes or compounds of Formula III and/or Formula IV and/or Formula V in order to produce pest control compositions, in particular insecticides, comprising such compounds.

Production of pyripyropenes from dry biomass

Microorganisms and processes for the recombinant manufacture of clavine-type alkaloids such as cycloclavine, festuclavine, agroclavine, chanoclavine and chanoclavine aldehyde, as well as polypeptides, polynucleotides and vectors comprising such polynucleotides which can be applied in a method for the manufacture of clavine-type alkaloids are provided.

Genes and processes for the production of clavine-type alkaloids

The invention pertains to the field of production of natural products and, in particular, in the field of production of cornexistin and hydroxycornexistin. It provides polynucleotides encoding polypeptides involved in the biosynthesis of cornexistin and hydroxycornexistin as well as vectors and recombinant microorganisms comprising such polynucleotides. Also provided are methods for the production of natural products, in particular methods for the production of cornexistin and hydroxycornexistin, using such polynucleotides and polpeptides encoded therein, as well as vectors and recombinant microorganisms comprising such polynucleotides and polypeptides.

Gene cluster for biosynthesis of cornexistin and hydroxycornexistin

The present invention relates to a method for preparing the pyripyropene compound of the formula (I) which method comprises the following steps: i) subjecting a pyripyropene compound of formula Pyripyropene A to an alkaline hydrolysis to yield a 1,7,11 -trideacetylpyripyropene A, ii) reacting 1,7,11-trideacetylpyripyropene A obtained in step i) with cyclopropane carbonyl chloride to yield a raw product containing the pyripyropene compound of formula (I); iii) subjecting the raw product of step ii) to crystallization to yield a crystalline pyripyropene compound of formula (I) and a mother liquor; and iv) recycling the mother liquor or a pyripyropene compound containing fraction thereof to the alkaline hydrolysis of step i). 

Method for preparing a pyripyropene compound

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Nussloch, Germany

Hartwig Schroeder