Basf Se Corporation

Basf Aktiengesellschaft

Evonik Degussa Gmbh

Hartwig Schroder

Stefan Haefner

Oskar Zelder

Corinna Klopprogge

Andrea Herold

Burkhard Kröger

Hansgeorg Ernst

R Rogers Yocum

Rajan Hollmann

Christoph Wittmann

Edzard Scholten

Gregory Von Abendroth

Elmar Heinzle

Mark K Williams

Hans Gurski

Aline Raddatz

Stephan Haefner

Patrick Kiefer

The present invention relates to a novel bacterial strain designated DD1, which has the ability to produce organic acids, in particular succinic acid (SA), which was originally isolated from bovine rumen, and is capable of utilizing glycerol as a carbon source; and variant strains derived there from retaining said capability; as well as to methods of producing organic acids, in particular succinic acid by making use of said microorganism.

Bacterial strain and fermentative process for producing succinic acid

The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.

PEF-TS expression units

The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.

Microbial succinic acid producers and purification of succinic acid

The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a &#x394;metF organism or a &#x394;metE AmetH organism, for example, mutants ofor, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A &#x394;metF &#x394;metB strain ofcan use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

Use of dimethyl disulfide for methionine production in microoraganisms

Psod expression units

The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g.,by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine inby way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

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P EF-TU expression units

The present invention relates to multiple promoters and to expression units comprising them; to the use thereof for regulating transcription and expression of genes; to expression cassettes which comprise multiple promoters or expression units of this kind; to vectors which comprise such expression cassettes; to genetically modified microorganisms which comprise vectors and/or expression units of this kind; and to processes for preparing biosynthetic products by culturing said genetically modified microorganisms.

Multiple promoters and the use thereof for gene expression

Growing community of inventors

Nussloch, Germany

Hartwig Schroder