Boehringer Mannheim Gmbh

Roche Diagnostics Gmbh

Hartmut Merdes

Ulfert Deneke

Walter Rittersdorf

Gerhard Hiller

Uwe Goebbert

Klaus Buecker

Dieter Heindl

Herbert Harttig

Hans-Peter Josel

Rupert Herrmann

Kurt Stach

Herbert Berger

Hans Lange

Rudi Gall

Hans Wielinger

Werner Guthlein

Rudolf Pachl

Wolfgang Vomel

Axel Schmidt

Manfred Kuhr

Robert Lorenz

Detlef Thym

Winfriede Sauer

Martina Junius-Comer

Werner Guethlein

Hans-Rudiger Murawski

Joachim Hones

Ernst Selbertinger

Wofgang-Reinhold Knappe

Manfred Zeiler

An apparatus comprising a first porous carrier and a second porous carrier for evaluating biological fluid samples is disclosed. The apparatus is used for separating non high density lipoprotein (non-HDL) from a lipoprotein in a body sample and for determining high density lipoprotein (HDL) cholesterol in a HDL and non high density lipoprotein (non-HDL) in a body sample.

Method for the determination of HDL cholesterol by means of a rapid diagnostic agent with an integrated fractionating step

The invention concerns the use of an asymmetric porous membrane for the evenly distributed concentration of a substance which is not or not substantially adsorbed to the membrane and which is brought into contact with the membrane in the form of a membrane-wetting solution on the fine-pored side of the membrane.

Asymmetric porous membranes

The subject matter of the application is a method for the separation of non-HDL lipoproteins from biological fluids in a carrier through which liquids can flow. The carrier contains a precipitating agent for non-high density lipoproteins (non-HDLs).

Method for separating non-HDLs from HDLs, and determining, HDL cholesterol

The invention concerns the use of redox-active compounds for the production of detection reagents for a method for the determination of an analyte as well as reagent kits that contain these redox-active compounds. In addition new redox-active compounds are disclosed.

Redox-active compounds and their use

The present invention concerns a diagnostic test carrier for the determination of an analyte from whole blood with the aid of a reagent system contained in the carrier which includes a colour forming reagent with a test field which has a sample application side onto which the blood sample is applied and a detection side on which an optically detectable change takes place as a result of the reaction of analyte with the reagent system and which is constructed in such a way that erythrocytes present in the sample do not reach the detection side, which is characterized in that the test field comprises a transparent foil onto which a first and a second film layer are applied on top of one another and wherein the first layer located on the transparent foil scatters light considerably less in a wet state than the overlying second layer and wherein the side of the foil on which the first layer is applied which is opposite to the foil side is the detection side and the side of the second layer which is opposite to the side with which the second layer rests on the first is the sample application side. In addition the invention concerns a method for the determination of an analyte from whole blood with the aid of a diagnostic test carrier according to the invention.

Diagnostic test carrier with multilayer field

The invention relates to a method useful in separating non-high density lipoproteins, referred to as 'non-HDLs', from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDL-s are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.

Method for separating non-high density lipoproteins from lipoprotein

The invention relates to a method useful in separating non-high density lipoproteins, referred to as 'non-HDLs', from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDLs are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.

Method for the determination of HDL cholesterol by means of a rapid

The invention relates to an apparatus and a method useful in separating non-high density lipoproteins, referred to as 'non-HDLs', from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDLs are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.

Apparatus for determination of HDL cholesterol

New 1-naphtholphthalein monophosphates of the formula ##STR1## wherein each of R.sup.1 and R.sup.2 is hydrogen or halogen, and each of M.sup.1 and M.sup.2 is a proton or an alkali metal, alkaline earth metal, ammonium, dicyclohexylammonium, tetraalkylammonium or alkanolammonium cation, and the tautomeric transformation products thereof, are useful as substrates for detection and photometric determination of alkaline phosphotase.

1-naphtholphthalein monophosphates and diagnostic reagents containing

The present invention provides imidazole derivatives of the general formula: ##STR1## wherein R, R.sub.1 and R.sub.2 are defined hereinbelow. The present invention is also concerned with processes for the preparation of these imidazole derivatives and with the use thereof as redox indicators.

Imidazole derivatives as redox indicators

Certain novel nitrofuran compounds of the formula: ##SPC1## Wherein

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Heidelberg, Germany

Hartmut Merdes