Los Alamos National Security, LLC

Triad National Security, LLC

Harshini Mukundan

Basil I Swanson

Jennifer Suzanne Martinez

Wynne Kevin Grace

Hongzhi Xie

Pulak Nath

Aaron S Anderson

Rama Murthy Sakamuri

Kiersten Danielle Lenz

A microfluidic device can include at least one separation unit defining a first chamber fluidly coupled to an opening and a trap chamber disposed downstream of the opening, and a second chamber that extends radially outwardly of the opening. The second chamber can be offset from the first chamber along a first axis of the device and along a second axis of the device. The separation unit can further comprise a membrane disposed between the first and second chambers and extending across the opening, the membrane being configured such that when a sample is received in the first chamber and the device is rotated, the sample flows across the membrane and filtrate passes through the membrane into the second reservoir and residue flows into the trap chamber.

Microfluidic device

Provided herein are systems for and methods of capturing, detecting, quantifying, and characterizing target moieties that are characterized by having a lipophilic portion of sufficient size and chemical composition whereby the target moiety inserts (or partitions) into a lipid assembly. Examples of such assays employ synthetic lipid constructs such as supported bilayers which are used to capture target moieties; other example assays exploit the natural absorption of compounds into natural lipid constructs such as HDL or LDL particles or cell membranes to capture target moieties. In specific embodiments, the target moieties are bacterial pathogen associated molecular pattern (PAMP) molecules or compounds not yet identified as PAMP molecules. Also provided are methods of determining PAMP molecule fingerprints and profiles that are linked to (indicative of) bacterial infection, disease states or progression, development of antibiotic resistance, and so forth, as well as these fingerprints, profiles and methods of using them.

Discovery, detection and use of biomarkers

The present invention addresses the simultaneous detection and quantitative measurement of multiple biomolecules, e.g., pathogen biomarkers through either a sandwich assay approach or a lipid insertion approach. The invention can further employ a multichannel, structure with multi-sensor elements per channel.

Quantitative multiplex detection of pathogen biomarkers

Growing community of inventors

Los Alamos, NM, United States of America

Harshini Mukundan