Cpc International, Inc.

Harry W Leach

Ronald E Hebeda

Dennis J Holik

Clarence F Harjes

John M Trp

Protease enzyme impurities contained in bacterial .alpha.-amylase enzyme preparations are inactivated by a mild heat treatment in the presence of a protective material. Useful protective materials include calcium and starch hydrolysates such as corn syrup. The protease-free .alpha.-amylase can then be used to solubilize starch materials by various granular starch and conventional processes. Hydrolysates obtained contain significantly less soluble protein than those prepared using untreated .alpha.-amylases. A preferred .alpha.-amylase enzyme preparation is one derived from a Bacillus licheniformis microorganism.

Protease inactivated .alpha.-amylase preparations

A process for production of dextrose from starch wherein a starch hydrolysate having a high dextrose equivalent produced using soluble glucoamylase is treated solely with an immobilized glucoamylase enzyme to produce a dextrose product.

Dextrose production with immobilized glucoamylase

A process for converting granular starch to a soluble hydrolysate wherein the unconverted starch retains its granular nature. The conversion is accomplished by means of a bacterial alpha-amylase.

Enzymatically produced maltose-maltotriose starch hydrolysate

A low D.E. starch hydrolysate having a D.E. not substantially above about 20, a relatively narrow molecular weight distribution such that its weight average molecular weight to its number average molecular weight ratio of Mw/Mn is less than about 20 and said hydrolysate being further characterized as containing less than about 20% by weight, dry basis, of starch oligosaccharides having a degree of polymerization greater than about 200. The low D.E. starch hydrolysates are prepared by enzymatically hydrolyzing starch dextrins having a degree of branching of at least about 7%. The low D.E. starch hydrolysates are resistant to the formation of haze at high solids and they exhibit reduced enzyme susceptability.

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Willowbrook, IL, United States of America

Harry W Leach