Promega Corporation

Harry Tetsuo Uyeda

Carolyn Woodroofe Hitko

Keith V Wood

Thomas A Kirkland

Dieter Klaubert

Poncho Meisenheimer

Rachel Friedman Ohana

Sergei Saveliev

Ji Zhu

Daniel J Simpson

Paul Otto

Nidhi Nath

Marjeta Urh

Robin Hurst

Wenhui Zhou

Joel R Walker

Sergiy Levin

Mary P Hall

Hui Wang

Ce Shi

Cesear Corona

Min Zhou

Zhiyang Zeng

Robert Kargbo

Valerie Ressler

Jun Zhang

Jean Osterman

Prabin Rai

The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.

Substrates for covalent tethering of proteins to functional groups or solid surfaces

The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.

Cleavable surfactants

Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.

Acid-labile surfactants

Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

Coelenterazine analogues

The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

Compositions and methods for capture of cellular targets of bioactive agents

The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

Described herein are antibacterial compounds, methods for making the compounds, pharmaceutical compositions containing the compounds and methods of treating bacterial infections utilizing the compounds and pharmaceutical compositions.

Luminogenic compounds and methods to detect cytochrome P450 3A enzymes

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Los Osos, CA, United States of America

Harry Tetsuo Uyeda