California Institute of Technology

Molecular Instruments, Inc.

Harry M T Choi

Niles A Pierce

Long Cai

Chun Hao R Chen

Aneesh Acharya

Naeem S Husain

Mike C Liu

The present disclosure relates to methods and compositions involving HCR reactions that involve initiators that are split into two or more parts. Effective HCR is dependent upon two or more of these split initiators being brought into proximity (e.g., via binding events mediated by a target) such that a full initiator is formed that is capable of triggering HCR signal amplification.

Fractional initiator hybridization chain reaction

The present application relates to hybridization chain reaction (HCR). In particular, compositions and methods are presented for ultrasensitive molecular detection using HCR signal amplification. Some embodiments and methods involve cooperative probe junctions, reporter-labeled probes, and nonlinear HCR signal amplification.

Ultrasensitive molecular detection via hybridization chain reaction

Growing community of inventors

Pasadena, CA, United States of America

Harry M T Choi