Abbott Laboratories Corporation

Harold N Baker

William R Groskopf

Gregg T Williams

Dalmacio A Agdeppa

Eugene W Osikowicz

Paula Trach

Michael K Massei

Katherine K Eng

Necklaws: Elizabeth C

William D Gurner

Sally K Ramp

Determination of steroids by competitive immunoassay

A method and kit for measurement of a steroid by means of a competitive immunoassay, preferably a competitive enzyme immunoassay. The method and kit involve the use of a steroid analogue conjugated to a label. The steroids that are amenable to detection by the method and kit of the present invention include estradiol and progesterone. The method comprises the steps of:

A method and kit for measurement of asteroid by means of a competitive immunoassay, preferably a competitive enzyme immunoassay. The method and kit involve the use of asteroid analogue conjugated to a label. The steroids that are amenable to detection by the method and kit of the present invention include estradiol and progesterone.

The present invention relates to the measurement of estradiol using competitive immunoassay methods. The inventors unexpectedly discovered that estrone and its derivatives conjugated to a label is a particularly effective tracer when used in conjunction with estradiol specific antibodies to determine estradiol levels in fluid samples. The present invention also utilizes 5.alpha.-dihydrotestosterone to enhance the assay performance.

Determination of estradiol by competitive immunoassay

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Libertyville, IL, United States of America

Harold N Baker