Other

Carter Wallace, Inc.

Center for Laboratory Medicine

Harold M Bates

Frank M Berger

Bernard J Ludwig

David B Reisner

Cody A Evans

An improved method of testing individuals for diabetes, even if they have levels of interfering substances (e.g., uric acid, bilirubin, and glutathione) that would otherwise interfere with such testing, is disclosed. The individual's protein-bound glucose level and glucose level are compared to the analogous values for a reference population to enable the risk of that individual's having diabetes to be assessed. The substances that would otherwise tend to interfere with the assay for protein-bound glucose are removed before the assay, desirably by precipitating the protein-bound glucose using uranyl acetate, which desirably leaves substantially all of the interfering substances in the supernatant, then separating the precipitate from the supernatant, redissolving the precipitate, and conducting the colorimetric assay on the resulting solution. An improved colorimetric test for protein-bound glucose using viologens as the colorimetric electron acceptors is also disclosed.

Method of testing for diabetes that reduces the effect of interfering

Chemical compounds of the formula: ##STR1## wherein X is hydrogen, hydroxy, halogen, lower alkyl, lower alkoxy, nitro, amino, acetamido, sulfonamido or trifluoromethyl, and each R is selected from the group consisting of hydrogen and lower alkyl. Said compounds have valuable anti-inflammatory activity in standard laboratory animals.

2,3,4,4A-Tetrahydro-10H-1,2-oxazino[3,2-b](1,3)benzoxazin-10-ones

Thyroid hormones in blood serum are determined by admixing a blood serum sample with pepsin, incubating the sample for a period of time to allow pepsin to digest proteins having a thyroid hormone bound thereto and release the thyroid hormone from the proteins, adjusting the resulting mixture to a pH at which pepsin is inactive and assaying the mixture for the released thyroid hormone.

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East Brunswick, NJ, United States of America

Harold M Bates