Unisyn Technologies, Inc.

Allergan, Inc.

Harish P Kumar

Terrence J Hunt

That T Ngo

Jack Z Xie

Huong T Tran

Don Mathewson

Ananda Seneviratne

Alex Praseuth

Gopal Dasari

A method for preparation of immunoglobulin fragments by specific fragmentation of immunoglobulins, comprising treating a solution of immunoglobulins with a soluble protease so as to achieve substantially complete fragmentation of the immunoglobulins, followed by complexing the protease with a complexing agent (for example, antibody to the protease) to form a protease complex and separating the protease complex from the immunoglobulin fragments. Optionally, the protease (e.g., pepsin or papain) is inactivated prior to formation of the protease complex. In contrast to methods which use immobilized forms of the enzymes, the rate of cleavage and the final yields of F.sub.(ab) and F.sub.(ab')2 fragments are increased substantially.

Method for preparing immunoglobulin fragments

Growing community of inventors

El Toro, CA, United States of America

Harish P Kumar