Pluristem Ltd.

Harel Kasuto

Eytan Abraham

Nirit Drori-carmi

Barak Zohar

Lior Raviv

Zami Aberman

Nirit Drori-Carmi

Ohad Karnieli

Dotan Hoffman

Methods for using vibration to harvest cells grown in 3D culture are provided. The methods entail the application of force cells attached to a 3D matrix of sufficient amplitude, frequency, and duration to detach cells from the matrix and to flush the detached cells out of the matrix material. An apparatus for performing the methods of the invention as provided.

Methods and systems for harvesting cells

This disclosure is directed to methods and systems for growing and/or harvesting cells. The method can include seeding cells on a plurality of carriers. The method can also include incubating the carriers under conditions suitable for cell growth.

Systems and methods for growing and harvesting cells

Carrier for cell culture

Methods for using vibration to harvest cells grown in 3D culture are provided. The methods entail the application of force to cells attached to a 3D matrix of sufficient amplitude, frequency, and duration to detach cells from the matrix and to flush the detached cells out of the matrix material. An apparatus for performing the methods of the invention as provided.

Devices and methods for culture of cells are provided. The devices can comprise a three-dimensional body having multiple two-dimensional surfaces extending inwardly from a periphery of the three-dimensional body towards an interior of the three-dimensional body, wherein the multiple two-dimensional surfaces are configured to support monolayer growth of eukaryotic cells over at least a majority of or all of the surface area of the multiple two-dimensional surfaces.

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Kibbutz Yifat, Israel

Harel Kasuto