Waters Technologies Corporation

Thar Technologies, Inc.

Thar Instruments, Inc.

Harbaksh Sidhu

Ziqiang Wang

Steven L Zulli

Lalit M Chordia

Daniel Rolle

Chuping Luo

Todd Palcic

John Whelan

A supercritical fluid chromatography system comprises a first pump for pumping a first flow stream comprising a compressible fluid and a second pump for pumping a second flow stream comprising a modifier fluid. The second pump is in parallel with the first pump. A column is located in a combined flow stream. The column is located downstream of the first and second pumps. The combined flow stream comprises the first flow stream, the second flow stream, and a sample. A detector is located downstream of the column. A gas-liquid separator is located downstream of the detector. The gas-liquid separator is configured to vent a majority of the compressible fluid while maintaining a majority of the sample, thus preventing aerosolization of the flow stream and minimizing sample loss as well as cross contamination. An open bed collector is located after the gas-liquid separator.

Methods and devices for open-bed atmospheric collection for supercritical fluid chromatography

A collection system for collecting samples from a flowstream () exiting a supercritical fluid chromatography system is provided. The collection system comprises: (i) a first back pressure regulator () on the flowstream as it exits the chromatography system, (ii) a gas-liquid separator () having a tapered and angled dripper (), which introduces the flow into the separator at an angle tangential to the separator wall; and (iii) one or more fraction collectors (), wherein the fraction collector is at a reduced pressure collection point, between 100 bar and atmospheric pressure. A collection system for HPLC is also provided, as well as a process for collection of samples from a flowstream.

Collection system for purification flowstreams

A method for separating a sample using high pressure flash chromatography is provided. The method comprises the steps of: i) providing a pressurized vessel containing an adsorption material; ii) pressurizing a compressible fluid, optionally containing a cosolvent, to create a mobile phase; iii) premixing the sample with the mobile phase or optionally placing the sample in the pressurized vessel; iv) eluting the mobile phase through the pressurized vessel, to obtain a separated sample; v) heating the mobile phase containing the separated sample after the mobile phase exits the pressurized vessel to remove the compressible fluid; and iv) collecting the separated sample. The pressurized vessel contains an adsorption material having a particle size of 10-100 microns, and the pressure of the adsorption vessel is held at 50-350 bar during elution. Also provided is an apparatus for carrying out the above method.

High pressure flash chromatography

The present invention provides a method and device for collecting and concentrating a desired compound from a chromatographic column exit stream. The collection chamber can be heated or cooled and washed in a forward or backward direction for efficient removal.

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Allison Park, PA, United States of America

Harbaksh Sidhu