The General Hospital Corporation

Iviva Medical, Inc.

Miromatrix Medical Inc.

Cilag International Gmbh

Regents of the University of Minnesota

Harald C Ott

Charles C Klassen

Doris Taylor

Maria Jaramillo

Robert G Hesse

Daniel Cheng

Xi Ren

Jordan P Bloom

Taufiek Konrad Rajab

Jason L Harris

Chad E Eckert

Jordan B Wong

Sarah E Gilpin

The present application provides methods of functionalizing an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal or by culturing an organ or tissue in a bioreactor containing such nutrient. The present application also provides methods of selectively functionalizing extracellular matrix (ECM) of an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal. In some aspects, the present application provides a decellularized scaffold of a mammalian organ or tissue comprising an extracellular matrix, wherein the extracellular matrix of the decellularized scaffold is functionalized with a chemical group that is reactive in a bioorthogonal chemical reaction, such as an azide chemical group. The present application also provides biological prosthetic mesh and mammalian organs and tissues for transplantation prepared according to the methods of the application.

Metabolic labeling and molecular enhancement of biological materials using bioorthogonal reactions

Disclosed are apparatus and methods for blood and other biological fluid purification using a membrane with cell containing vascular channel systems and filtration channel systems. Also disclosed are methods of making the apparatus as well as methods of making membranes.

Apparatus for supplementing function of endocrine glands

Presented is an airway organ bioreactor apparatus, and methods of use thereof, as well as bioartificial airway organs produced using the methods, and methods of treating subjects using the bioartificial airway organs. The bioreactor comprises: an organ chamber: an ingres line connecting the organ chamber and a reservoir system and comprising an arterial line, a venous line and a tracheal line; an egress line connecting the chamber and the reservoir system, pumps in ingress and egress lines; a controller to control fluid exchange; a chamber pressure sensor connected to the organ chamber.

Lung bioreactor

Biological fluid purification with biocompatible membranes

In general, systems, methods, and devices for modeling air leaks in lungs are provided. In an exemplary embodiment, the systems, methods, and devices provide a model to allow for simulation of negative and positive pressure ventilation modes in vitro, which may allow for evaluation and investigation of pathologies and interventions. In addition to ventilation functions the model includes submersion of the lung sample in fluid inside a chamber and cycling the fluid through a collection system to collect air leaked from the lung. The model can allow for quantification and visual observation of air leaks in real time.

Modeling air leaks in lungs

Presented is an airway organ bioreactor apparatus, and methods of use thereof, as well as bioartificial airway organs produced using the methods, and methods of treating subjects using the bioartificial airway organs. The bioreactor comprises: an organ chamber; an ingres line connecting the organ chamber and a reservoir system and comprising an arterial line, a venous line and a tracheal line; an egress line connecting the chamber and the reservoir system, pumps in ingress and egress lines; a controller to control fluid exchange; a chamber pressure sensor connected to the organ chamber.

A method for vascular regeneration comprises delivering endothelial cells to a lung scaffold, delivering perivascular cells to the lung scaffold, and providing a multiphase culture program to the scaffold. The multiphase culture program comprises a first phase including delivering an angiogenic medium, e.g., having 40-100 ng/ml of pro-angiogenic factors, and a second phase including delivering a stabilization medium, e.g., having 0.5-2% of serum and 1-20 ng/ml of angiogenic factors.

Regeneration of a functional pulmonary vascular bed

Methods of using human airway stem cells in lung epithelial engineering, optionally wherein the cells are contacted with a gamma secretase inhibitor, bioartificial airway organs produced thereby, and the use thereof, e.g., for transplantation. Also methods of treating a bio-artificial matrix with Tenascin-C and/or fibrillin 2.

Human airway stem cells in lung epithelial engineering

The invention provides for methods and materials to decellularize a solid organ and to recellularize such a decellularized organ to thereby generate a solid organ.

Decellularization and recellularization of solid organs

Presented is an airway organ bioreactor apparatus, and methods of use thereof, as well as bioartificial airway organs produced using the methods, and methods of treating subjects using the bioartificial airway organs.

Growing community of inventors

Wenham, MA, United States of America

Harald C Ott