Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Basf Corporation

Danstar Ferment Ag

Universiteit Stellenbosch

Haowen Xu

John E McBride

Allan C Froehlich

Emily Stonehouse

Mark Mellon

Riaan Den Haan

Kristen M Deleault

John S Bardsley

Aaron Argyros

Elena E Brevnova

Erin Wiswall

Indraneel Shikhare

Trisha Barrett

Nicky Caiazza

Ryan Skinner

Heidi H Hau

Kevin S Wenger

Jennifer Gosselin

Alan Belcher

Vineet Rajgarhia

Merja Elisa Penttila

Merja Penttilä

Charles F Rice

Anne K Warner

Chhayal V Gandhi

Shital A Tripathi

Frank Agbogbo

Yin-Ying Chiu

Abigail Foster

Vineet B Rajgarhia

Alan Benjamin Gilbert

John E Mcbride

Matti Siika-Aho

Marja Ilmen

Sanni Voutilainen

Jaana Uusitalo

Anu Koivula

Deidre Willes

Jim Flatt

Emily A Stonehouse

Dan Murphy

Heidi Hanson Hau

Jeffrey D Keating

Jeff Villari

Chhayal Ghandi

Alan Froehlich

Sharad Mathur

Arthur J Shaw, Iv

William Ryan Sillers

David Anthony Hogsett

Emile Van Zyl

Danie Lagrange

Elena Brevnova

Karin Arens

Steve Licht

Hans Van Dijken

Naomi Thorngren

Heidi Lau

Merja Penttila

Arthur J Shaw Iv

Abigail S Foster

Shaunita Rose

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present application relates to reaction mixtures comprising biomass, a solid acid catalyst and cellulolytic enzymes or organisms expressing such enzymes for converting biomass to useful feedstocks and methods of forming products via fermentation using the said reaction mixture.

Converting biomass to fermentative products

The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability ofto produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose

The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.

Expression of enzymes in yeast for lignocellulose derived oligomer CBP

One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.

Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate

Host cells, comprisingexpressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.

Growing community of inventors

Lebanon, NH, United States of America

Haowen Xu