Mgi Tech Co., Ltd.

Bgi Genomics Co., Limited

Haojun Jiang

Lin Yang

Hui Fu Jiang

Fang Zhou Chen

Yanyan Zhang

Ya Gao

Jing Guo

Xun Xu

Peng Zeng

Yuqian Wang

Shengpei Chen

Fang Chen

Weiwei Xie

Chunlei Zhang

Guodong Huang

Haiping Zhang

Xuehan Zhuang

Shaohong Zhang

Yifan Xie

Jing Zheng

Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3' end of the first primer, the first random sequence is located at the 5′ end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3′ end of the second primer, the second random sequence is located at the 5′ end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.

PCR primer pair and application thereof

Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3' of the first primer, and the first random sequence is located on end 5&#x2032; of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3&#x2032; of the second primer, and the second random sequence is located on end 5&#x2032; of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.

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Shenzhen, China

Haojun Jiang