Arryx, Inc

Haojun Fu

Christopher R Knutson

Osman Akcakir

Daniel Mueth

Gary R Stacey

Kenneth Forbes Bradley

Evan Tanner

Matthew K Runyon

Robert Lancelot

Tania Chakrabarty

Dan Mueth

Crystal J Duke

Sergio O Guevara

The present invention relates to methods and apparatuses for the detection of positional freedom of particles used in biological, biochemical, physical, biophysical, and chemical analyses. In particular, the present invention relates to methods and apparatuses which can detect and characterize a population of particles/cells based upon their detected mobility. In one embodiment consistent with the invention, detection of certain cells is based on differences detected in populations of cells that bind to a substrate and those that exhibit weaker binding forces. Initially, cells are settled on the substrate, and in the presence of gravitational, natural thermodynamic pressure fluctuations, and other random or applied forces, some of the particles may exhibit translational movement. Particle movement is detected, and measurements are computed, according to the methods and apparatuses of the present invention, to determine the binding of specific analytes.

Methods and apparatuses for detection of positional freedom of particles in biological and chemical analyses and applications in immunodiagnostics

A rotor is provided for use in a centrifuge system configured to spin the rotor for separating complex fluids. The rotor includes a housing configured to be secured by the centrifuge system and several chambers formed in the housing. Each chamber includes a first chamber portion having a port formed therein and a second chamber portion in fluid communication with the port of the first chamber portion. The second chamber portion may be disposed generally below the port of the first chamber portion. Other rotor designs and methods for separating complex fluids are further disclosed.

Centrifuge rotor for separation and processing of complex fluids

Methods and apparatus for analyzing surface properties of particles are provided. A method for analyzing the surface properties of the particle includes a associating a first particle with a first capture zone having a specific binding affinity for a first chemical species, applying an optical force to the first particle, sensing a response of the first particle to the optical force, and using the sensed response to determine the presence, absence or quantity of the first chemical species on the first particle surface. This process may be repeated in parallel to test multiple particles. In addition to directly testing the surface properties of the particles, the method can be used in direct, indirect and competitive assays to determine the presence, absence or quantity of free or immobilized analytes. A fluidic cartridge with capture zones having avidities that are tuned for the use of optical forces is provided. A software routine for performing the method is also provided.

Surface mapping by optical manipulation of particles in relation to a functionalized surface

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Naperville, IL, United States of America

Haojun Fu