Sap Se

Abelzeta Inc.

Shanghai Cellular Biopharmaceutical Group Ltd.

Shanghai Abelzeta Ltd.

Haojie Zhang

Fang Liu

Ting Yan

Jiangguo Ying

Yi Hong

Luyi Zhang

Jun Wu

Fei Wang

Xiao Min Xu

Weiyi Cui

Dijun Zhao

Jinghui Li

Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (l) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

Method for large-scale production of lentivirus by using GMP-level serum-free suspension cells

Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

Method for large-scale preparation of purified preparation of recombinant lentiviral vector at GMP grade

Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (1) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

A method for value-added usage of process-oriented extension fields in business mashups may include generating, using at least one processor, a mashup component with an unbound parameter; receiving input identifying the creation of an extension field, the extension field tied to data stored in a database; binding the unbound parameter of the mashup component to the extension field; presenting an application interface, the application interface including the extension field; receiving a request to load the mashup component; and in response to receiving the request, passing the data tied to the extension field as an input parameter to the mashup component.

Value-added usage of process-oriented extension fields in business mashups

Growing community of inventors

Shanghai, China

Haojie Zhang