Tencent Music Entertainment Technology (shenzhen) Co., Ltd.

Bontac Bio-engineering (shenzhen) Co., Ltd

Haojie Xu

Ge Tang

Qi Zhang

Xiang Cao

Qin Wang

Zhang Zhang

Zhaoheng Lei

Shuo Zhao

Guiping Zhang

Xiaozhen Ji

Zhengtao Wang

The invention relates to a method for preparing nicotinamide mononucleotide by using nicotinamide as a raw material, which comprises: in acetonitrile, dichloromethane, 1,2-dichloroethane or liquid sulfur dioxide as a solvent, allowing nicotinamide and tetraacetyl ribose to react as catalyzed by trimethylsilyl trifluoromethanesulfonate or tin tetrachloride, adjusting a pH value thereof to 3-5, adding a sodium methoxide solution thereto to react at −15° C. to 5° C., adjusting a pH value thereof to 3-5, and subjecting the reaction mixture to microfiltration and nanofiltration using a membrane concentrator, thereby obtain a nicotinamide ribose solution; allowing the nicotinamide ribose solution to react as catalyzed by nicotinamide ribokinase in the presence of Mg ions, ATP and a buffer, thereby obtaining nicotinamide mononucleotide. The method of the invention omits the step of refining nicotinamide ribose, and thus has simpler process, lower cost and less time consumption, and has the advantages of faster reaction speed and lower enzyme consumption compared with the enzyme catalytic process directly using refined nicotinamide ribose solid.

Method for preparing nicotinamide mononucleotide by using nicotinamide as raw material

Provided a method for playing audios. The method includes: acquiring vibration control information corresponding to a target audio, wherein at least one vibration period and vibration attribute information corresponding to the at least one vibration period are recorded in the vibration control information, and each vibration period corresponds to a beat period of a target percussive instrument in the target audio; synchronously playing the target audio and the vibration control information; and when any vibration period of the at least one vibration period is played, controlling a terminal to vibrate based on vibration attribute information corresponding to the vibration period.

Method and system for playing audios

Provided is a method for playing media data. The method includes: receiving a trigger instruction; generating a second count value based on a stored first count value and a preset algorithm, and storing the second count value to overwrite the first count value, wherein the second count value is different from the first count value; generating audio data carrying a pre-stored authority identifier and the second count value; and playing the audio data, wherein the audio data is configured to instruct a second terminal to acquire the authority identifier and the second count value carried in the audio data, and send the authority identifier and the second count value to a server, and the second terminal is a terminal that has received the audio data.

Method, apparatus and system for playing media data, and device and storage medium

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Shenzhen, China

Haojie Xu