Berkeley Lights, Inc.

Bruker Cellular Analysic, Inc.

Hansohl E Kim

John A Tenney

Thomas M Vetterli

Peter J Beemiller

Yelena Bronevetsky

Natalie C Marks

Guido K Stadler

Vincent Haw Tien Pai

Joshua Foster Slocum

Annamaria Mocciaro

Jason M Mcewen

Amanda L Goodsell

Duane Smith

Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.

Methods for assaying biological cells in a microfluidic device

Methods are provided for the automated detection, characterization, and selection of micro-objects in a microfluidic device. In addition, methods are provided for grouping detected micro-objects into subgroups that share the same characteristics and, optionally, repositioning micro-objects in a selected sub-population within the microfluidic device. For example, micro-objects in a selected sub-population can be moved into sequestration pens. The methods also provide for visual displays of the micro-object characteristics, such as two- or three-dimensional graphs, and for user-based definition and/or selection of sub-populations of the detected micro-objects. In addition, non-transitory computer-readable medium in which a program is stored and systems for carrying out any of the disclosed methods are provided.

Automated detection and characterization of micro-objects in microfluidic devices

Methods are provided for the automated detection and/or counting of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.

Automated detection and repositioning of micro-objects in microfluidic devices

Growing community of inventors

Emeryville, CA, United States of America

Hansohl E Kim