The University of British Columbia

Hans Zahn

Carl Lars Genghis Hansen

Jens Huft

Sohrab Shah

Marinus Theodorus Johannes Van Loenhout

Kaston Leung

Bill Kengli Lin

Anders Klaus

Samuel Alves Jana Rodrigues Aparicio

Adi Steif

Anupam Singhal

Carl L G Hansen

Daniel Jay Da Costa

Veronique Lecault

Marketa Ricicova

Kevin Albert Heyries

Oleh Petriv

Brad Nelson

Julie Nielsen

Kathleen Lisaingo

Sohrab Prakash Shah

Carl L Hansen

Methods, devices and systems for analyzing precious samples of cells, including single cells are provided. The methods, devices, and systems in various embodiments of the invention are used to assess genomic heterogeneity, which has been recognized as a central feature of many cancers and plays a critical role in disease initiation, progression, and response to treatment. The methods devices and systems are also used to analyze embryonic biopsies for preimplantation genetic diagnosis (PGD). In one embodiment, the devices, systems and methods provided herein allow for the construction of genomic and RNA-seq libraries without a pre-amplification step.

Methods for analyzing genomic variation within cells

Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.

Microfluidic devices and methods for use thereof in multicellular assays of secretion

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Munich, Germany

Hans Zahn