Centre Suisse D'electronique Et De Microtechnique Sa

Csem Centre Suisse D'electronique Et De Microtechnique Sa

Forschungszentrum Karlsruhe Gmbh

Arrayon Biotechnolgy Sa

Hans Sigrist

Dieter Lohmann

Rino Ernst Kunz

Carsten Korth

Michael Rapp

Jean Gobet

Bruno Oesch

Markus Moser

Nicole Barie

J&uuml;rg D&uuml;bendorfer

Yann Chevolot

Hans-J&ouml;rg Mathieu

Jose Martins

David Crout

Hui Gao

Hendrik Van Den Vlekkert

Hui Chai-Gao

Nigel Skinner

In a dextran coated surface disposed on a carrier, the connections between the dextran and the carrier surface are formed by a photolinker, the dextran coating being attached to the carrier surface by co-immobilization of a mixture of dextran and the photolinker.

Dextran-coated surface

The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater then 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method o end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labelled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labelling can be accomplished by providing a nucleic acid, providing labelled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.

Biochemical sensor system with increased sensitivity by molecular amplification of signal

Process for the modification of surface

This document describes an optical sensor unit and a procedure for the specific detection and identification of biomolecules at high sensitivity in real fluids and tissue homogenates. High detection limits are reached by the combination of i) label-free integrated optical detection of molecular interactions, ii) the use of specific bioconstituents for sensitive detection and iii) planar optical transducer surfaces appropriately engineered for suppression of non-specific binding, internal referencing and calibration. Applications include the detection of prion proteins and identification of those biomolecules which non-covalently interact with surface immobilized prion proteins and are intrinsically involved in the cause of prion related disease.

Optical sensor unit and procedure for the ultrasensitive detection of chemical or biochemical analytes

The invention relates to a device including a substrate and at least one biologically active substance bound to at least a part of the surface of this substrate, which is obtained by simultaneous or sequential reaction of said substrate and of said substance with a bifunctional coupling agent in which one of the functional groups is capable of being photoactivated and generates carbenes and is used to bind the coupling agent to the inorganic substrate and the other functional group is used to bind the coupling agent to the biologically active substance, in which said substrate is a covalent inorganic nitride.

Device including a biologically active substance immobilized on a

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Kernenried, Switzerland

Hans Sigrist