Boehringer Mannheim Gmbh

Hans Seidel

Helmgard Gauhl

Wolfgang Gruber

Albert Roder

Klaus Beaucamp

Hans Mollering

Peter Stahl

Joachim Siedel

Herwig Brunner

Ulrich Mayr

Detlef Von Hoerschelmann

Herbert Von Der Eltz

Gunter Lang

Michael Nelboeck

Joachim Ziegenhorn

Alfred Mertens

Herbert Leinert

Otto-Henning Wilhelms

Harald Zilch

Wolfgang Vomel

Androniki Roesch

Peter Roeschlau

Michael Nelboeck-Hochstetter

Bernhard Konig

Friedrich Gotz

Ulrike Leser

Karl-Heinz Schleifer

Erich Elstner

Barbara Sedewitz

Georg Schawohl

Gerhard Berger

Armin Masuth

Friedrich Giffhorn

Use of thiazoloisoindolinone derivatives as antiviral medicaments

The present invention provides a process for obtaining sarcosine oxidase from micro-organisms by culturing thereof and obtaining the enzyme from the biomass or from the culture broth, wherein a Pseudomonas strain is cultured.

Process for obtaining sarcosine oxidase from microorganisms

The present invention provides a method of use for a hydrogen peroxide-forming oxidase, wherein the enzyme is obtained from Streptomycetaceae and at 25.degree. C., in 0.15 mol/liter potassium phosphate (pH 7.9), in the presence of surface-active substances, still shows after 2 days an activity of at least 40% of the initial activity. This enzyme is useful in the determination of sarcosine, creatine and creatinine.

Method for the determination of sarcosine creatine or creatinine

Pyrrothine derivatives and pharmaceutical use thereof

The present invention provides a hydrogen peroxide-forming sarcosine oxidase, wherein it is obtainable from Streptomycetaceae and at 25.degree. C. in 0.15 mol/liter potassium phosphate (pH 7.9), in the presence of surface-active substances, still shows after 2 days an activity of at least 40% of the initial activity.

Hydrogen peroxide-forming sarcosine oxidase

The present invention provides a new pyruvate oxidase which decarboxylates pyruvate with the formation of hydrogen peroxide, characterized in that it is active without the addition of FAD, TPP and divalent metal ions. The present invention also provides a process for preparing this new enzyme and a reagent containing it.

Pyruvate oxidase

The present invention provides a process for obtaining cholesterol esterase from micro-organisms by culturing a micro-organism capable of forming cholesterol esterase in an appropriate nutrient medium in the presence of an inductor and obtaining the enzyme from the culture liquid and/or from the cells, therein the inductor used is a compound of the general formula: ##STR1## in which R and R.sub.1 are alkyl or alkoxy radicals containing 14 to 18 carbon atoms and R or R.sub.1 can also be a hydrogen atom and R.sub.2 is an alkylamino radical containing 2 to 8 carbon atoms, an alkyl-trimethylammonium radical containing 3 to 8 carbon atoms, an alkylpyridine radical containing up to 4 carbon atoms in the alkyl moiety or a radical of the general formula --CH.sub.2 --(CHOH).sub.n --CH.sub.2 OH, in which n is a whole number of from 1 to 4.

Process for obtaining cholesterol esterase from microorganisms

A L(+)-tartrate dehydrogenase with L(+)-tartrate dehydrogenase and D(+)-malate dehydrogenase (decarboxylating) activity, which can be obtained from Rhodopseudomonas sphaeroides, and also a process for the production thereof. Furthermore, a process and a reagent for the determination of L(+)-tartrate and of D(+)-malate.

Determination of L(+)-tartrate or D(+)-malate with L(+)-tartrate

The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a colored material is formed from the decarboxylation product in the presence of tyrosinase by oxidative coupling with an appropriate colored material component, the colored material formed then being determined photometrically. The present invention also provides a reagent for the determination of NADH or NADPH, wherein it contains salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer, as well as a reagent for the determination of salicylate, wherein it contains NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.

Determination of NAD(P)H or salicylate

For the determination of glycerin by oxidation with oxygen in the presence of glycerinoxidase and measurement of the oxygen consumption or of the H.sub.2 O.sub.2 formation, a glycerinoxidase from Aspergillus spec. DSM 1729 is used. A reagent suitable for this method consists of glycerinoxidase from Aspergillus spec. DSM 1729 and a system for the determination of H.sub.2 O.sub.2 and contains additionally, if desired, an agent for the saponification of esterified glycerin.

Method and reagent for the determination of glycerin

The present invention provides a process for obtaining glucose dehydrogenase by culturing a micro-organism in an appropriate nutrient medium and obtaining the enzyme from the cell mass or from the culture medium, wherein the micro-organism cultured is Bacillus cereus DSM 1644. The nutrient medium used contains a carbon source which consists predominantly or solely of glycerol.

Process for obtaining glucose dehydrogenase and micro-organism therefor

Process for obtaining high yields of glycerol dehydrogenase from micro-organisms and glycerol dehydrogenase having a low K.sub.M value.

Process for the recovery of glycerol dehydrogenase

The present invention provides a process for the determination of .beta.-lactamases by the use of 7-cyanoacetylaminocephalosporanic acid and measurement of the colored material thereby formed, wherein ammonium ions are added to the material to be tested, together with phosphate ions and/or an agent splitting off oxygen. The present invention also provides a reagent for the determination of .beta.-lactamases based upon 7-cyanoacetylaminocephalosporanic acid, which reagent additionally contains ammonium ions, together with phosphate ions and/or an agent splitting off oxygen.

Reagent for the determination of .beta.-lactamase

The present invention provides a process for obtaining cholesterol esterase from micro-organisms of the genus Pseudomonas, wherein Pseudomonas sp. DSM 1280 or DSM 1281 is cultured in an appropriate culture medium in the presence of an inducer and the enzyme is obtained from the culture medium and/or from the cells.

Process for the preparation of cholesterol esterase

The present invention provides a process for obtaining cholesterol esterase from micro-organisms, wherein a micro-organism capable of cholesterol esterase formation is cultured in an appropriate nutrient medium in the presence of lecithin as inducer and the enzyme is obtained from the culture medium and/or from the cells.

Growing community of inventors

Tutzing, Germany

Hans Seidel