Biotest-serum-institut Gmbh

Biotest Pharma Gmbh

Biotest-serum Institute Gmbh

Hans Schleussner

Wolfgang Stephan

Herbert Dichtelmuller

Wolfram H Walker

Dieter Rudnick

Wolfgang Moller

Norbert Kothe

Detlef Piechaczek

Udo Schwulera

Hans H Sonneborn

Karlheinz Ganshirt

Horst Uthemann

Dieter Merz

Reinhard Fiebig

Karl H Ganshirt

Alexander Thrun

A method of sterilizing plasma or plasma fractions, including fractions that contain the blood-coagulating Factor VIII by treatment with .beta.-propiolactone or ultraviolet radiation. Treatment with tri-n-butyl phosphate and sodium cholate or Tween 80 is carried out either prior to or simultaneously with the .beta.-propiolactone treatment or ultraviolet radiation.

Method of sterilizing plasma or plasma fractions

A method of producing a virus-safe, storage-stable, and intravenously tolerable immunoglobulin-G preparation. The object is to make the method appropriate for industrial-scale production and economical by means of the enrichment and multistage purification of a plasma that has had the coagulation factors removed from it or of a plasma fraction or serum fraction that contains immunoglobulin G accompanied by treatment with ion exchangers and by ultrafiltration. The precipitant is eliminated, by means of diafiltration or gelfiltration, either from the plasma that has had the coagulation factors removed from it or from the plasma fraction that contains the immunoglobulin G, and the desired ion composition is established. The resulting protein solution is subjected to fractionation over an ion exchanger to separate the immunoglobulin G. The proteolytic enzymes in the resulting immunoglobulin-G solution are removed by means of affinity chromatography over a dye linked sorbent and/or are ihibited by the addition of antithrombin III. The accordingly stabilized immunoglobulin-G solution is treated, at a protein concentration of 20 to 60 g/l and a pH 6.0 to 8.0, with 0.03 to 0.07% of .beta.-propiolactone, diluted to a protein concentration of 5 to 20 g/l and subjected to ultraviolet radiation. The accordingly sterilized dilute immunoglobulin-G solution is again diafiltered or gelfiltered to remove lower molecular-weight substances. The accordingly stabilized and sterilized solution is adjusted to the desired protein content of 2 to 16% and filtered sterile.

Method of producing a virus safe, storage-stable, and intravenously

A microtiter plate for blood typing consisting of a flat-bottomed plate of rigid transparent polystyrene having a plurality of wells, the polystyrene being surface treated by a corona and/or plasma method of applying high power electric energy at high frequency resulting in a permanently measurable negative ion-charge at the surface of the wells, and layers of substantially pure antiserums dried on the wells without any binder from a suspension or solution of the antiserums and tightly but releasably bound to the polystryene.

Microtiter plate for blood typing

A compositon for treating diseases which comprises a therapeutically effective amount of an antibacterial, antiviral, or antimycotic agent, antitrypsin, antithrombin, interferon, or lysozyme, and an immunoenhancing amount of immunoglobulins IgG, IgM, and IgA, and a method of therapy of a mammal in need thereof are disclosed.

Combination of immunoglobulins with therapeutic agents

Crude interleukin-2 extract is subjected to group-selective dye-ligand absorption chromatography in one or more stages of purification with a matrix-gel medium consisting of a Blue A ligand or variant thereof or of a Green A ligand in a concentration of approximately 1.5 to 3.0 mg/ml of expanded matrix at a pH of approximately 6.8 to 8.5, a temperature of approximately 4.degree. to 40.degree. C., and a flowthrough rate of approximately 10 to 100 ml/h, employing an eluent. Either PHA-free or extremely pure interleukin-2 is obtained, depending on the overall number of purification stages.

Separation of interleukin-2 from phytohemagglutinin by dye matrix

A bag suitable for the storage, deep-freezing and transfusion of blood and blood components formed of a calendered or extruded foil of plasticizer-free polyurethane of a thickness of about 0.1-0.5 mm, a Shore hardness A of about 60-85, temperature resistance up to about 160.degree. C. and down to about -196.degree. C. without change of properties. The bag can be used in combination with fittings and tubings of the same material to provide an overall plasticizer-free polyurethane system. The bag can be sterilized, is capable of low temperature storage, and exhibits other desirable properties without affecting the quality of its contents.

Polyurethane bag for blood

A microfiltration device for the filtration of coagula and microaggregates from blood and blood components. The device contains a filter chamber with attaching nozzle, a filter cone containing truncoconical filter elements arranged in cascade with diminishing pore size and diminishing surface area, a drip chamber and a tailpiece. The filter chamber and filter cones have different tapers and are at a specific distance apart, the taper of the filter cone from inlet to outlet being greater than the taper of the filter chamber. The filter cone has up to four filter elements, and a spacer which extends into the filter element of smallest pore size and surface area and supports same. Each of the filter elements has at least two opposed ribs running from the base to the apex of the truncated cone. The spacer is constructed such that it serves simultaneously for the support of the ribs and for the support of the filter fabric of the smallest filter element. The device has a capacity of less than 100 ml. It is intended for use in transfusions of preserved blood or blood components, especially massive transfusions.

Microfiltration device for the filtration of coagula and microaggregates

A nutrient medium carrier system comprising a sterilizable plastic nutrient medium carrier, a sterilizable plastic cover, and a sealing foil, the nutrient medium carrier having a depression to receive the nutrient medium, a surrounding beaded edge above the surface of the nutrient medium, a handle on one end, and a flat edge running around both sides and the other end, the depression being subdivided into compartments by means of ridges; the cover having a depression and a surrounding flat edge to receive the sealing foil, the cover depression gradually widening on two sides and one end to a flat edge up to the dimensions of the nutrient medium carrier, the narrow part being of such size that both side walls and one end touch the beaded edge on two sides and on the end opposite the handle, the cover having a steplike offset with locking flanges on its upper edge to hold the nutrient medium carrier, the flat edge of the carrier contacting the steplike offset of the cover, the cover on the side on which the handle of the nutrient medium carrier comes to lie being rounded off or slanted and provided with a hollow for gripping and pulling out the nutrient medium carrier from the cover.

Growing community of inventors

Frankfurt am Main, Germany

Hans Schleussner