Basf Plant Science Gmbh

Scandinavian Biotechnology Research (scanbi) Ab

Hans Ronne

Marit Lenman

Antoni Banas

Sten Stymne

Anders Dahlqvist

Ulf Stahl

Martijn Gipmans

Eva Wiberg

Andres Dahlqvist

Sten Stymme

Line Sandager

Ulf St&aring;hl

Ulf Stähl

The present invention relates to the isolation, identification and characterization of nucleotide sequences encoding an enzyme catalysing the transfer of fatty acids from phospholipids to diacylglycerol in the biosynthetic pathway for the production of triacylglycerol, to said enzymes and process for the production of triacylglycerols.

Process for the production of triacylglycerols

The invention relates to methods for increasing the oil content in plants, preferably in plant seeds, by expressing a polypeptide from yeast. The invention furthermore relates to expression constructs for expressing the yeast polypeptide in plants, preferably in plant seeds, the transgenic plants expressing the yeast polypeptide and to the use of said transgenic plants for the production of food, feeds, seed, pharmaceuticals or fine chemicals, in particular for the production of oils.

Use of a gene for increasing the oil content in plants

The present invention relates to the production of triacylglycerol in a transformed organism or host cell, by means of a nucleotide sequence fromwhereby said enzyme catalyzes the transfer of fatty acids from phospholipids to diacylglycerol in any acyl-CoA-independent reaction. The transformation of the organism or host cell results in increased oil content.

Methods of making triacylglycerol using phospholipid:Diacylglycerol acyltransferase

The present invention relates to the use of a novel enzyme and its encoding gene for transformation. More specifically, the invention relates to the use of a gene encoding an enzyme with acyl-CoA:diacylglycerol acyltransferase activity. This gene expressed alone in transgenic organisms will increase the total amount of oil (i.e. triacylglycerols) that is produced.

Use of a class of enzymes and their encoding genes to increase the oil content in transgenic organisms

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Uppsala, Sweden

Hans Ronne