Pharma-Zentrale Gmbh

Pharma-Zentral

Hans Proppert

Ulrich Sonnenborn

J&uuml;rgen Malinka

J&uuml;rgen Schulze

J&ouml;rg Hacker

Gabriele Blum-Oehler

Tobias Oelschlaeger

Sibylle Oswald

Ernst Theodor Rietschel

Ulrich Z&auml;hringer

Artur Ulmer

Gabrielle Blum-Oehler

Ulrich Sonnen-Born

The invention relates to a plasmid-free clone ofstrain DSM 6601, to a method for preparing the same and to the use thereof as a cloning vehicle.

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Lipopolysaccharides and processes for producting the lipopolysaccharides are provided. The lipopolysaccharide has a lipid A portion, a core oligosaccharide portion, and an O-specific chain having a single repeating unit 06. A lipopolysaccharide may be produced by washing and drying and bacterial mass, subjecting the washed and dried bacterial mass to a phenol/water extraction, and treating the extract with RNases, DNases, and proteinase K.

Lipopolysaccharides (LPS) extracted from Escherichia coli

Methods for identifying strain DSM 6601 and nucleotide sequences associated therewith.

Method for identifying Escherichia coli strain DSM 6601

plasmids pMUT 1 and pMUT 2 containing DNA sequences exhibiting significant homologies to reduplicaton starting positions of other enterobacterial plasmids are disclosed. Also disclosed are expression vectors containing plasmicis pMUT1 and pMUT 2.

Bacterial plasmids

The invention relates to DNA sequences from fimbrial gene clusters of strain DSM 6601. This strain has two chromosomal fimbrial gene clusters, namely type I (fim) and FIC (foc) gene clusters. DNA sequences of the main subunits fimA and focA are represented by SEQ ID NOS: 1 and 2.

DNA sequences of genes from fimbriae d'escherichia coli strain DSM 6601

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Hagen, Germany

Hans Proppert