The United States of America, As Represented by the Secretary, Department of Health and Human Services

Hans Peter Hinrikson

Christine J Morrison

Novel techniques for the detection ofin samples are disclosed. These techniques relate to PCR amplification and/or detection ofITS1 rDNA sequences, and the identification of particular species ofby detecting differences in the ITS1-V1, ITS-V2, ITS-V3, ITS-V4, and ITS-V5 nucleic acid sequences of. The highly variable regions of the ITS1 rDNA sequences are particularly useful in distinguishing, for example,, and. In particular embodiments, the sequence differences are also able to distinguish among variants of particular species, such asCBS 119.5A,strain NRRL 1932,strain NRRL 250,strain NRRL 4768,strain CUHI,strain CUH2,strain CUH7, andstrain CUH8.

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Wallisellen, Switzerland

Hans Peter Hinrikson