Bayer Aktiengesellschaft

Bayer Healthcare LLC

Aicuris Gmbh + Co. Kg.

Hans-Juergen Henzler

Klaus Kaiser

Rolf Treckmann

Joerg Kauling

Cynthia J Galloway

Kathryn Remington

Michael Pohlscheidt

Berthold Boedeker

Heiner Apeler

Uwe Langer

Katrin Brabender

Dirk Otto-Brabender

Joachim Kerper

A method of inactivating microorganisms such as viruses within a fluid such as a biological fluid is disclosed. The method includes the steps of providing a UV reactor, which may take the form of an elongated generally annular reaction chamber surrounding at least one elongated UV lamp, moving the fluid within the reaction chamber in a primary flow directed along the length of the UV lamp, and inducing a circulating secondary flow within the fluid with the secondary flow being superimposed on the primary flow. As the fluid moves through the reaction chamber in the primary flow, it is circulated repeatedly toward and away from the UV lamp in the circulating secondary flow to provide uniform and controllable exposure of the entire volume of fluid to ultraviolet radiation. Microorganisms such as viruses are thus inactivated while desirable components in the fluid, such as proteins, are preserved without the use of a free radical scavenger.

Method of inactivating microorganisms in a fluid using ultraviolet radiation

The invention relates to a method for preparing viral suspensions. The invention relates in particular to a method for preparing high-titer viral suspensions in cell cultures. Preferred methods include increasing the volume of the cell culture prior to infection with viral material and subsequent further steps of expanding the volume to a final volume which is distinctly larger than the maximum culture volume prior to infection.

Method for preparing viral material

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Solingen, Germany

Hans-Juergen Henzler