Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Alaeris Theranostics Gmbh

Hans-Joerg Warnatz

Hans Lehrach

Joern Gloekler

The invention relates to a method for linking at least two target nucleic acid molecules from a single biological compartment, comprising the steps of isolating a fraction from a sample, wherein the fraction comprises the compartment comprising at least two nucleic acid molecules, diluting the fraction and aliquoting the dilution in multiple separate reaction vessels such that each reaction vessel comprises preferably one compartment, or encapsulating the compartment in emulsion droplets such that each droplet comprises preferably one compartment; linking the at least two target nucleic acid molecules, preferably by overlap extension PCR. The method may be employed in the analysis of mutations present in a single cell and in the production of antibodies which are present in a single hybridoma.

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Berlin, Germany

Hans-Joerg Warnatz