Mount Sinai School of Medicine

Icahn School of Medicine at Mount Sinai

Hans Joerg Fehling

Gordon Keller

Valerie Kouskoff

Atsushi Kubo

Paul Gadue

Stefan Irion

Herve Luche

Gordon M Keller

The invention provides a method for generating a transgenic eukaryotic cell population having a modified human Rosa26 locus, which method includes introducing a functional DNA sequence into the human Rosa26 locus of starting eukaryotic cells. Also provided are targeting vectors useful in the method, as well as a cell population and a transgenic non-human animal comprising a modified human Rosa26 locus. Finally, the invention provides an isolated DNA sequence corresponding to the human Rosa26 locus.

Vector for targeting the human Rosa26 gene

The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. In accordance with the present invention, a selectable marker gene has been recombinantly targeted to the brachyury locus to allow the isolation and characterization of cell populations that comprise brachyury positive mesendoderm and mesoderm cells. The cell populations of the invention are useful for generating cells for cell replacement therapy.

Method of generating human pancreatic cells

Stem cell gene targeting

Targeting the Rosa26 gene in human embryonic stem cells

The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy.

Cell population enriched for endoderm cells

Method of enriching a mammalian cell population for mesoderm cells

Mesoderm and definitive endoderm cell populations

Growing community of inventors

Ulm, Germany

Hans Joerg Fehling