Cargill Inc.

Novozymes A/s

Akzo Nobel N.v.

United States of America, As Represented by the Secretary of Agriculture

Lynch, Michael

Copley, Shelley

Louie, Michael

Hans H Liao

Holly J Jessen

Eileen Spindler

Olga V Selifonova

Steven John Gort

Ravi R Gokarn

Wendy Kathleen Ribble

Michael Tai Man Louie

Michael David Lynch

Travis Robert Wolter

Tanya Lipscomb

Ana Negrete-Raymond

Christopher Patrick Mercogliano

Catherine B Poor

Ron Evans

Brittany Robinson

Shelley Copley

Matthew Lipscomb

Brittany Prather

Michael Louie

Steffen Schaffer

Aristos Aristidou

Pirkko Suominen

Michael D Lynch

Erin Kathleen Marasco

Tanya E W Lipscomb

Liv Reinecke

Jian Yi

Jan Wolter

Katrin Grammann

Joshua Lundorff

David A Hogsett

Ronald J Evans

Tanya Lipscomb-Warnecke

Dave A Hogsett

Bob Van Gemen

Chris Miller

Victor J Chan

Akio Sugiyama

Dominic W S Wong

Meiling Shang

Mary J Zidwick

Joseph R Warner

Tristan Lipkie

David Hogsett

Chrisopher Patrick Mercogliano

Eileen Colie Spindler

Tanya Lipscomp

Dave Hogsett

Brittany L Prather

Tanya Warnecke Lipscomb

Eileen C Spindler

Holly Jean Jessen

Disclosed herein are pigment compositions for meat substitutes and meat substitutes including such pigment compositions. The pigment compositions include a thermolabile EforRed variant polypeptide. The pigment compositions provide a pink and/or red color to a meat substitute composition that transitions to a brown color after cooking. For example, the thermolabile EforRed variant may have a sequence at least 80% identical to SEQ ID NO:1 and include (i) an XYG chromophore tripeptide and (ii) a mutation at a position selected from the group consisting of phenylalanine (F) 68, threonine (T) 102, aspartate (D) 119, D130, alanine (A) 139, F163, F206, A214, and combinations thereof relative to SEQ ID NO:1.

Thermolabile pigments for meat substitutes derived by mutation of the pigment of coral

This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.

Microorganisms and methods for the production of fatty acids and fatty acid derived products

Genes encoding mutant 3-ketoacyl-CoA synthases are introduced into host cells. Certain of the mutants enhance the production of shorter-chain fatty acids and derivatives by the cell than do the wild-type (unmutated) enzymes. In other cases, the chain length is not significantly affected, but productivity is enhanced. In specific cases, both a shift toward lower chain length and higher productivity is seen. Cells producing the mutant 3-ketoacyl-CoA synthases are especially suitable for producing C6-C10 fatty acids and derivatives.

Genetically modified cells that produce C6-C10 fatty acid derivatives

The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.

Acetyl-CoA carboxylases

A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.

Production of fatty acids esters

Bioproduction of chemicals

The present application discloses the identification of the novelxylose transporter genes KHT105 and RAG4, as well as the identification of a novel set ofpentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.

Compositions and methods for increased ethanol production from biomass

Acetyl-coA carboxylases

Growing community of inventors

Eden Prairie, MN, United States of America

Hans H Liao